論文 - 詳細
RRC ID | 43769 |
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著者 | Tanabe R, Cha BY, Ha BG, Yonezawa T, Nagai K, Woo JT, Yagasaki K. |
タイトル | Detection of adiponectin and monocyte chemoattractant protein-1 using a calixcrown derivatives-coated protein chip. |
ジャーナル | J Immunoassay Immunochem |
Abstract |
We used a ProteoChip coated with a calixcrown derivative protein linker to measure adiponectin and monocyte chemoattractant protein-1 (MCP-1) levels and compared the results with commercial enzyme-linked immunosorbent assay (ELISA) kits. Adiponectin and MCP-1 levels in normal human serum and RAW264 cell supernatants, respectively, were measured. The ProteoChip quantification results correlated with those from the ELISA kits; however, the ProteoChip required less sample volume, exhibited higher sensitivity, and had a wider detection range. The ProteoChip was capable of detecting and quantifying small amounts of protein, possibly replacing ELISA kits in evaluating the levels of adiponectin and MCP-1. |
巻・号 | 33(2) |
ページ | 166-79 |
公開日 | 2012-1-1 |
DOI | 10.1080/15321819.2011.615360 |
PMID | 22471607 |
MeSH | Adiponectin / analysis* Adiponectin / immunology Animals Antibodies, Monoclonal / immunology Calixarenes / chemistry* Calixarenes / immunology Cell Line Chemokine CCL2 / analysis* Chemokine CCL2 / immunology Crown Ethers / chemistry* Crown Ethers / immunology Enzyme-Linked Immunosorbent Assay Humans Mice Protein Array Analysis / instrumentation Protein Array Analysis / methods* Recombinant Proteins / analysis Recombinant Proteins / immunology Sensitivity and Specificity |
引用数 | 0 |
WOS 分野 | BIOCHEMICAL RESEARCH METHODS IMMUNOLOGY MEDICAL LABORATORY TECHNOLOGY |
リソース情報 | |
ヒト・動物細胞 | RAW 264(RCB0535) |