RRC ID |
43986
|
著者 |
Jin G, Mizutani A, Fukuda T, Chen L, Nakanishi K, Yan T, Kudoh T, Hirohata S, Kasai T, Murakami H, Salomon DS, Seno M.
|
タイトル |
Eosinophil cationic protein enhances cardiomyocyte differentiation of P19CL6 embryonal carcinoma cells by stimulating the FGF receptor signaling pathway.
|
ジャーナル |
Growth Factors
|
Abstract |
We investigated the functional role of eosinophil cationic protein (ECP) in regulating cardiomyogenesis using mouse P19CL6 embryonic carcinoma cells. ECP was confirmed to accelerate the cardiomyocyte differentiation of P19CL6 cells by enhancing the rate and area size of beating of cardiomyocyte and by facilitating the expression of cardiomyocyte-specific genes, such as GATA4 and α-MHC. Since cardiomyocyte differentiation in vivo is considered to follow mesoderm induction, the induction of Brachyury, a marker of mesoderm, was assessed. Brachyury expression was found to be enhanced after the addition of ECP. This enhancement was due to the stimulation of extracellular signal-regulated kinase (ERK)1/2 phosphorylation by ECP. In this context, treatment with SU5402, an inhibitor of fibroblast growth factor (FGF) receptor 1, suppressed Brachyury expression, phosphorylation of ERK1/2, and cardiomyocyte differentiation induced by ECP. We concluded that ECP might induce mesoderm differentiation through FGF signaling pathway and enhance subsequent cardiomyocyte differentiation in concert with dimethyl sulfoxide in P19CL6 cells. ECP may be a novel factor for cardiomyocyte differentiation, which should be very useful to prepare adequate numbers of cardiomyocytes for therapeutic cell transplantation.
|
巻・号 |
30(5)
|
ページ |
344-55
|
公開日 |
2012-10-1
|
DOI |
10.3109/08977194.2012.709852
|
PMID |
22845717
|
MeSH |
Animals
Cell Differentiation
Cell Line, Tumor
Dimethyl Sulfoxide / pharmacology
Embryonal Carcinoma Stem Cells / cytology*
Embryonal Carcinoma Stem Cells / metabolism
Eosinophil Cationic Protein / metabolism*
Extracellular Signal-Regulated MAP Kinases / metabolism
Fetal Proteins / biosynthesis
GATA4 Transcription Factor / biosynthesis
Mice
Myocytes, Cardiac / cytology*
Myocytes, Cardiac / metabolism*
Myosin Heavy Chains / biosynthesis
Phosphorylation
Protein-Tyrosine Kinases / antagonists & inhibitors
Pyrroles / pharmacology
Receptors, Fibroblast Growth Factor / antagonists & inhibitors
Receptors, Fibroblast Growth Factor / metabolism*
Signal Transduction
T-Box Domain Proteins / biosynthesis
|
IF |
1.543
|
引用数 |
2
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
CELL BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
P19CL6 |