論文 - 詳細
| RRC ID | 44140 |
|---|---|
| 著者 | Kubiura M, Okano M, Kimura H, Kawamura F, Tada M. |
| タイトル | Chromosome-wide regulation of euchromatin-specific 5mC to 5hmC conversion in mouse ES cells and female human somatic cells. |
| ジャーナル | Chromosome Res |
| Abstract |
DNA cytosine methylation (5mC) is indispensable for a number of cellular processes, including retrotransposon silencing, genomic imprinting, and X chromosome inactivation in mammalian development. Recent studies have focused on 5-hydroxymethylcytosine (5hmC), a new epigenetic mark or intermediate in the DNA demethylation pathway. However, 5hmC itself has no role in pluripotency maintenance in mouse embryonic stem cells (ESCs) lacking Dnmt1, 3a, and 3b. Here, we demonstrated that 5hmC accumulated on euchromatic chromosomal bands that were marked with di- and tri-methylated histone H3 at lysine 4 (H3K4me2/3) in mouse ESCs. By contrast, heterochromatin enriched with H3K9me3, including mouse chromosomal G-bands, pericentric repeats, human satellite 2 and 3, and inactive X chromosomes, was not enriched with 5hmC. Therefore, enzymes that hydroxylate the methyl group of 5mC belonging to the Tet family might be excluded from inactive chromatin, which may restrict 5mC to 5hmC conversion in euchromatin to prevent nonselective de novo DNA methylation. |
| 巻・号 | 20(7) |
| ページ | 837-48 |
| 公開日 | 2012-10-1 |
| DOI | 10.1007/s10577-012-9317-9 |
| PMID | 23111490 |
| PMC | PMC3524505 |
| MeSH | 5-Methylcytosine / analogs & derivatives Animals Bromodeoxyuridine / metabolism Cell Line Chromosomes, Human, X / genetics Cytosine / analogs & derivatives* Cytosine / metabolism DNA (Cytosine-5-)-Methyltransferase 1 DNA (Cytosine-5-)-Methyltransferases / genetics DNA (Cytosine-5-)-Methyltransferases / metabolism DNA Methylation* Embryonic Stem Cells / cytology* Epigenesis, Genetic* Euchromatin / genetics* Euchromatin / metabolism Female Gene Deletion Gene Expression Regulation Gene Silencing Genetic Markers Genomic Imprinting Heterochromatin / genetics Heterochromatin / metabolism Histones / genetics Histones / metabolism Humans In Situ Hybridization, Fluorescence Male Mice Retroelements / genetics Sequence Analysis, DNA |
| IF | 3.413 |
| 引用数 | 24 |
| WOS 分野 | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |