| RRC ID |
44397
|
| Author |
Ishikawa H, Awano N, Fukui T, Sasaki H, Kyuwa S.
|
| Title |
The protective effects of lactoferrin against murine norovirus infection through inhibition of both viral attachment and replication.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The purpose of this study was to evaluate the effects of bovine lactoferrin against norovirus infection using mouse norovirus (MNV) and Raw264.7 cell in vitro. When Raw264.7 cells were infected with MNV in the presence or absence of lactoferrin, the cytotoxic damage to the infected Raw264.7 cells significantly and dose-dependently decreased and completely inhibited in the presence of 15 or 20 μg/well of lactoferrin as compared with the absence of lactoferrin. Correspondingly, the MNV titers in the culture medium and intracellularly were significantly decreased in infected Raw264.7 cells treated with lactoferrin compared to control infected Raw264.7 cells. The mechanisms responsible for the protective effects of lactoferrin against MNV infection were attributed to both its inhibition of the initial MNV attachment to cells and the subsequent interference with MNV replication. Moreover, it was revealed that lactoferrin could rapidly induce the expression of anti-viral cytokine mRNA, such as IFN-α and IFN-β which involved in inhibition of MNV replication in infected Raw264.7 cells, in the early phase of infection. It was concluded that lactoferrin exerts protective effects against MNV infection through inhibition of both viral attachment and replication. The present results provide evidence that lactoferrin may be useful as a preventive and/or therapeutic anti-norovirus agent.
|
| Volume |
434(4)
|
| Pages |
791-6
|
| Published |
2013-5-17
|
| DOI |
10.1016/j.bbrc.2013.04.013
|
| PII |
S0006-291X(13)00639-6
|
| PMID |
23603257
|
| MeSH |
Animals
Anti-Infective Agents / pharmacology
Antibodies, Neutralizing / immunology
Antibodies, Neutralizing / pharmacology
Cattle
Cell Line
Cell Survival / drug effects
Cell Survival / genetics
Dose-Response Relationship, Drug
Gene Expression / drug effects
Host-Pathogen Interactions / drug effects
Interferon-alpha / genetics
Interferon-alpha / immunology
Interferon-beta / genetics
Interferon-beta / immunology
Lactoferrin / pharmacology*
Macrophages / drug effects*
Macrophages / metabolism
Macrophages / virology
Mice
Norovirus / genetics
Norovirus / growth & development*
Norovirus / physiology
RNA, Viral / genetics
RNA, Viral / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Virus Attachment / drug effects*
Virus Replication / drug effects*
|
| IF |
2.985
|
| Times Cited |
14
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
