Reference - Detail
| RRC ID | 44789 |
|---|---|
| Author | Yamagiwa T, Inokuchi S, Saito T, Inoue S, Morita S, Kawaguchi AT. |
| Title | A stable in vitro method for assessing the toxicity of potassium cyanide and its antidote. |
| Journal | Tokai J Exp Clin Med |
| Abstract |
BACKGROUND:Hydrogen cyanide possesses a high acid-dissociation constant of 9.14, favoring its vaporization and depletion from the culture media at physiological pH, which may cause the cyanide toxicity unstable in vitro. OBJECTIVE:We investigated whether adjustment of culture medium pH stabilizes cyanide concentration and decreases the effective concentration of potassium cyanide (KCN). METHODS:Murine fibroblast cells were exposed to different concentrations of KCN in media maintained at pH 7.4 or 9.2, in the presence or absence of hydroxocobalamin. After incubation for 1 h, we evaluated medium pH, cyanide concentration, cytochrome activity, and cell viability. RESULTS:Cyanide concentration decreased to 18.8% in pH 7.4 medium compared to 83.2% in pH 9.2 medium. A significant decrease in cytochrome activity was observed at 40 mM and 1.25 mM KCN in pH 7.4 and pH 9.2 media, respectively. In pH 9.2 medium, dose-dependent cytotoxicity of KCN and antidotal effects of hydroxocobalamin were observed. CONCLUSION:Adjustment of culture medium pH to 9.2 could stabilize cyanide concentration and decrease the effective concentration of KCN, allowing stable evaluation of KCN toxicity and antidotal efficacy. |
| Volume | 38(4) |
| Pages | 114-22 |
| Published | 2013-12-20 |
| PMID | 24318282 |
| MeSH | Animals Antidotes / pharmacology* Cell Survival / drug effects* Cells, Cultured Culture Media / chemistry* Cytochromes / metabolism Dose-Response Relationship, Drug Fibroblasts / drug effects* Fibroblasts / enzymology Hydrogen-Ion Concentration Hydroxocobalamin / pharmacology* Mice Potassium Cyanide / analysis* Potassium Cyanide / toxicity* Toxicity Tests / methods* Volatilization |
| Resource | |
| Human and Animal Cells | NIH3T3 |