| RRC ID |
44792
|
| 著者 |
Takayama-Ito M, Nakamichi K, Kinoshita H, Kakiuchi S, Kurane I, Saijo M, Lim CK.
|
| タイトル |
A sensitive in vitro assay for the detection of residual viable rabies virus in inactivated rabies vaccines.
|
| ジャーナル |
Biologicals
|
| Abstract |
Rabies is a viral disease transmitted through bites from rabid animals and can be prevented by vaccines. Clinically used rabies vaccines are prepared from inactivated rabies viruses grown in cell cultures or embryonated eggs. In Japan and across the world, tests that confirm complete inactivation, such as the in vivo suckling mouse assay, in which suckling mice are intracerebrally inoculated with vaccine products, are required for quality control. In this study, we developed a novel cell-based immunofluorescence assay that does not require mice for testing rabies vaccine inactivation for human use. The sensitivity of this cell-based in vitro assay was 5.7 times that of the in vivo suckling mouse assay, with a detection limit of one focus forming units per ml of test sample. This newly developed in vitro assay may replace the established in vivo suckling mouse assay for confirming viral vaccine inactivation.
|
| 巻・号 |
42(1)
|
| ページ |
42-7
|
| 公開日 |
2014-1-1
|
| DOI |
10.1016/j.biologicals.2013.11.003
|
| PII |
S1045-1056(13)00135-8
|
| PMID |
24321529
|
| MeSH |
Animals
Animals, Suckling
Cell Line
Cricetinae
Humans
In Vitro Techniques
Mice
Rabies Vaccines / chemistry*
Vaccines, Inactivated / chemistry*
|
| IF |
1.801
|
| 引用数 |
2
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
|
