論文 - 詳細
| RRC ID | 44913 |
|---|---|
| 著者 | Tsuchiya A, Kanno T, Shimizu T, Nakao S, Tanaka A, Nishizaki T. |
| タイトル | DCP-LA-phosphatidylinositol and its enantiomer exhibit different bioactivities. |
| ジャーナル | Cell Physiol Biochem |
| Abstract |
BACKGROUND/AIMS:The present study was conducted to understand biochemical and biological characteristics of the phosphatidylinositol (PI) derivative 1,2-O-bis-[8-{2-(2-pentyl-cyclopropylmethyl)-cyclopropyl}-octanoyl]-Sn-glycero-3-phosphatidyl-D-1-inositol (diDCP-LA-PI) and its enantiomer 1,2-O-bis-[8-{2-(2-pentyl-cyclopropylmethyl)-cyclopropyl}-octanoyl]-Sn-glycero-3-phosphatidyl-L-1-inositol (diDCP-LA-PIe), with 8-[2-(2-pentyl-cyclopropylmethyl)-cyclopropyl]-octanoic acid (DCP-LA) on the α and β position. METHODS:Activities of protein kinase C (PKC) and protein phosphatases such as protein phosphatase 1 (PP1), PP2A, and protein tyrosine phosphatase 1B (PTP1B) were assayed under the cell-free conditions and in PC-12 cells. Akt1/2 activity was monitored by quantifying phosphorylation at Thr308/309 and Ser473/474 in PC-12 cells. RESULTS:diDCP-LA-PI significantly activated PKCα, -βΙ, -δ, and -ε, to an extent greater than that for diDCP-LA-PIe. diDCP-LA-PI still activated PKC in PC-12 cells, with the potential higher than that for diDCP-LA-PIe. Both diDCP-LA-PI and diDCP-LA-PIe reduced PP1 activity to a similar extent (30% of basal levels). diDCP-LA-PI enhanced PP2A activity to 180% of basal levels, while diDCP-LA-PIe had no effect. Drastic inhibition of PTP1B was obtained with diDCP-LA-PI and diDCP-LA-PIe, the extent reaching nearly 0% of basal levels. diDCP-LA-PI and diDCP-LA-PIe increased phosphorylation of Akt1/2 at Thr308/309 and Ser473/474 in PC-12 cells in the presence and absence of the PP2A inhibitor okadaic acid, respectively. CONCLUSION:The results of the present study show that diDCP-LA-PI and diDCP-LA-PIe exhibit different bioactivities with the different potentials each other. |
| 巻・号 | 33(2) |
| ページ | 300-9 |
| 公開日 | 2014-1-1 |
| DOI | 10.1159/000356670 |
| PII | 000356670 |
| PMID | 24525749 |
| MeSH | Animals PC12 Cells Phosphatidylinositols* / chemical synthesis Phosphatidylinositols* / chemistry Phosphatidylinositols* / pharmacology Phosphoprotein Phosphatases / metabolism* Phosphorylation / drug effects Protein Kinases / metabolism* Rats |
| IF | 5.5 |
| 引用数 | 2 |
| WOS 分野 | PHYSIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |