| RRC ID |
44973
|
| 著者 |
Ahtiainen L, Lefebvre S, Lindfors PH, Renvoisé E, Shirokova V, Vartiainen MK, Thesleff I, Mikkola ML.
|
| タイトル |
Directional cell migration, but not proliferation, drives hair placode morphogenesis.
|
| ジャーナル |
Dev Cell
|
| Abstract |
Epithelial reorganization involves coordinated changes in cell shapes and movements. This restructuring occurs during formation of placodes, ectodermal thickenings that initiate the morphogenesis of epithelial organs including hair, mammary gland, and tooth. Signaling pathways in ectodermal placode formation are well known, but the cellular mechanisms have remained ill defined. We established imaging methodology for live visualization of embryonic skin explants during the first wave of hair placode formation. We found that the vast majority of placodal cells were nonproliferative throughout morphogenesis. We show that cell compaction and centripetal migration are the main cellular mechanisms associated with hair placode morphogenesis and that inhibition of actin remodeling suppresses placode formation. Stimulation of both ectodysplasin/NF-κB and Wnt/β-catenin signaling increased cell motility and the number of cells committed to placodal fate. Thus, cell fate choices and morphogenetic events are controlled by the same molecular pathways, providing the framework for coordination of these two processes.
|
| 巻・号 |
28(5)
|
| ページ |
588-602
|
| 公開日 |
2014-3-10
|
| DOI |
10.1016/j.devcel.2014.02.003
|
| PII |
S1534-5807(14)00096-3
|
| PMID |
24636260
|
| MeSH |
Animals
Blotting, Western
Cell Cycle
Cell Movement*
Cell Proliferation*
Ectodysplasins / physiology
Embryo, Mammalian / cytology*
Embryo, Mammalian / metabolism
Female
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Hair Follicle / cytology*
Hair Follicle / embryology
Hair Follicle / metabolism
Image Processing, Computer-Assisted
Keratins / physiology*
Mice
Mice, Transgenic
Microscopy, Confocal
Microscopy, Fluorescence
Morphogenesis / physiology*
NF-kappa B / metabolism
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Wnt Proteins / metabolism
beta Catenin / metabolism
|
| IF |
10.092
|
| 引用数 |
54
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
