RRC ID |
45113
|
著者 |
Ikari A, Watanabe R, Sato T, Taga S, Shimobaba S, Yamaguchi M, Yamazaki Y, Endo S, Matsunaga T, Sugatani J.
|
タイトル |
Nuclear distribution of claudin-2 increases cell proliferation in human lung adenocarcinoma cells.
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ジャーナル |
Biochim Biophys Acta
|
Abstract |
Claudin-2 is expressed in human lung adenocarcinoma tissue and cell lines, although it is absent in normal lung tissue. However, the role of claudin-2 in cell proliferation and the regulatory mechanism of intracellular distribution remain undefined. Proliferation of human adenocarcinoma A549 cells was decreased by claudin-2 knockdown together with a decrease in the percentage of S phase cells. This knockdown decreased the expression levels of ZONAB and cell cycle regulators. Claudin-2 was distributed in the nucleus in human adenocarcinoma tissues and proliferating A549 cells. The nuclear distribution of ZONAB and percentage of S phase cells were higher in cells exogenously expressing claudin-2 with a nuclear localization signal than in cells expressing claudin-2 with a nuclear export signal. Nuclear claudin-2 formed a complex with ZO-1, ZONAB, and cyclin D1. Nuclear distribution of S208A mutant, a dephosphorylated form of claudin-2, was higher than that of wild type. We suggest that nuclear distribution of claudin-2 is up-regulated by dephosphorylation and claudin-2 serves to retain ZONAB and cyclin D1 in the nucleus, resulting in the enhancement of cell proliferation in lung adenocarcinoma cells.
|
巻・号 |
1843(9)
|
ページ |
2079-88
|
公開日 |
2014-9-1
|
DOI |
10.1016/j.bbamcr.2014.05.017
|
PII |
S0167-4889(14)00202-X
|
PMID |
24907662
|
MeSH |
Adenocarcinoma / metabolism*
Adenocarcinoma / pathology*
Adenocarcinoma of Lung
CCAAT-Enhancer-Binding Proteins / metabolism
Cell Cycle Proteins / metabolism
Cell Line, Tumor
Cell Nucleus / drug effects
Cell Nucleus / metabolism*
Cell Proliferation / drug effects
Claudins / metabolism*
Colforsin / pharmacology
Cyclin D1 / metabolism
G1 Phase / drug effects
Gene Knockdown Techniques
Heat-Shock Proteins / metabolism
Humans
Lung Neoplasms / metabolism*
Lung Neoplasms / pathology*
Mutant Proteins / metabolism
Nuclear Export Signals
Nuclear Localization Signals / metabolism
Phosphorylation / drug effects
S Phase / drug effects
Time Factors
Zonula Occludens-1 Protein / metabolism
|
IF |
3.411
|
引用数 |
38
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
A549 |