論文 - 詳細
| RRC ID | 45199 |
|---|---|
| 著者 | Milovac D, Gamboa-Martínez TC, Ivankovic M, Gallego Ferrer G, Ivankovic H. |
| タイトル | PCL-coated hydroxyapatite scaffold derived from cuttlefish bone: in vitro cell culture studies. |
| ジャーナル | Mater Sci Eng C Mater Biol Appl |
| Abstract |
In the present study, we examined the potential of using highly porous poly(ε-caprolactone) (PCL)-coated hydroxyapatite (HAp) scaffold derived from cuttlefish bone for bone tissue engineering applications. The cell culture studies were performed in vitro with preosteoblastic MC3T3-E1 cells in static culture conditions. Comparisons were made with uncoated HAp scaffold. The attachment and spreading of preosteoblasts on scaffolds were observed by Live/Dead staining Kit. The cells grown on the HAp/PCL composite scaffold exhibited greater spreading than cells grown on the HAp scaffold. DNA quantification and scanning electron microscopy (SEM) confirmed a good proliferation of cells on the scaffolds. DNA content on the HAp/PCL scaffold was significantly higher compared to porous HAp scaffolds. The amount of collagen synthesis was determined using a hydroxyproline assay. The osteoblastic differentiation of the cells was evaluated by determining alkaline phosphatase (ALP) activity and collagen type I secretion. Furthermore, cell spreading and cell proliferation within scaffolds were observed using a fluorescence microscope. |
| 巻・号 | 42 |
| ページ | 264-72 |
| 公開日 | 2014-9-1 |
| DOI | 10.1016/j.msec.2014.05.034 |
| PII | S0928-4931(14)00310-5 |
| PMID | 25063118 |
| MeSH | Alkaline Phosphatase / metabolism Animals Bone and Bones / chemistry* Cell Culture Techniques / instrumentation* Cell Line Cell Physiological Phenomena / drug effects Collagen / analysis Decapodiformes Durapatite / chemistry* Durapatite / pharmacology Mice Osteoblasts Polyesters / chemistry* Tissue Engineering / instrumentation Tissue Scaffolds / chemistry* |
| IF | 5.88 |
| 引用数 | 36 |
| WOS 分野 | MATERIALS SCIENCE, BIOMATERIALS |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |