RRC ID |
45225
|
著者 |
Maeda E, Tsutsumi T, Kitamura N, Kurokawa T, Ping Gong J, Yasuda K, Ohashi T.
|
タイトル |
Significant increase in Young's modulus of ATDC5 cells during chondrogenic differentiation induced by PAMPS/PDMAAm double-network gel: comparison with induction by insulin.
|
ジャーナル |
J Biomech
|
Abstract |
A double-network (DN) gel, which was composed of poly(2-acrylamido-2-methylpropanesulfonic acid) and poly(N,N'-dimethyl acrylamide) (PAMPS/PDMAAm), has the potential to induce chondrogenesis both in vitro and in vivo. The present study investigated the biomechanical and biological responses of chondrogenic progenitor ATDC5 cells cultured on the DN gel. ATDC5 cells were cultured on a polystyrene surface without insulin (Culture 1) and with insulin (Culture 2), and on the DN gel without insulin (Culture 3). The cultured cells were evaluated using micropipette aspiration for cell Young's modulus and qPCR for gene expression of chondrogenic and actin organization markers on days 3, 7 and 14. On day 3, the cells in Culture 3 formed nodules, in which the cells exhibited an actin cortical layer inside them, and gene expression of type-II collagen, aggrecan, and SOX9 was significantly higher in Culture 3 than Cultures 1 and 2 (p<0.05). Young's modulus in Culture 3 was significantly higher than that in Culture 1 throughout the testing period (p<0.05) and that in Culture 2 on day 14 (p<0.01). There was continuous expression of actin organization markers in Culture 3. This study highlights that the cells on the DN gel increased the modulus and mRNA expression of chondrogenic markers at an earlier time point with a greater magnitude compared to those on the polystyrene surface with insulin. This study also demonstrates a possible strong interrelation among alteration of cell mechanical properties, changes in actin organization and the induction of chondrogenic differentiation.
|
巻・号 |
47(13)
|
ページ |
3408-14
|
公開日 |
2014-10-17
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DOI |
10.1016/j.jbiomech.2014.07.021
|
PII |
S0021-9290(14)00402-3
|
PMID |
25110167
|
MeSH |
Acrylamides / chemistry*
Actin Cytoskeleton / drug effects
Actin Cytoskeleton / metabolism
Biomarkers / metabolism
Cell Differentiation / drug effects*
Cell Line, Tumor
Chondrocytes / cytology
Chondrocytes / drug effects
Chondrogenesis / drug effects*
Elastic Modulus / drug effects*
Gels
Gene Expression Regulation / drug effects
Humans
Insulin / pharmacology*
Polymers / chemistry*
Polymers / pharmacology*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sulfonic Acids / chemistry*
Sulfonic Acids / pharmacology*
|
IF |
2.32
|
引用数 |
3
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
BIOPHYSICS
|
リソース情報 |
ヒト・動物細胞 |
|