| RRC ID |
45300
|
| 著者 |
Sano K, Iwamiya Y, Kurosaki T, Ogawa M, Magata Y, Sasaki H, Ohshima T, Maeda M, Mukai T.
|
| タイトル |
Radiolabeled γ-polyglutamic acid complex as a nano-platform for sentinel lymph node imaging.
|
| ジャーナル |
J Control Release
|
| Abstract |
We established a ternary anionic complex constructed with polyamidoamine dendrimer (4th generation; G4) modified with chelating agents (diethylenetriamine pentaacetic acid (DTPA) derivative), polyethyleneimine (PEI), and γ-polyglutamic acid (PGA) as a safe nano-platform for molecular imaging. We prepared indium-111-labeled DTPA-G4/PEI/γ-PGA, and evaluated the effectiveness as a nuclear imaging probe for sentinel lymph node (LN), the first LN that drains the primary tumor. (111)In-DTPA-G4/PEI with strong cationic charge agglutinated with erythrocytes and showed extremely high cytotoxicity. By contrast, the anionic (111)In-DTPA-G4/PEI/γ-PGA had little agglutination activity with erythrocytes and no cytotoxicity, indicating their high biocompatibility. (111)In-DTPA-G4/PEI/γ-PGA was highly taken up by macrophage cells (high populations in LNs) comparable to (111)In-DTPA-G4/PEI. The uptake mechanisms of (111)In-DTPA-G4/PEI/γ-PGA were suggested to be both phagocytosis and γ-PGA-specific pathway. Upon administration of each (111)In-labeled nano-platform into rat footpads intradermally, significantly higher radioactivity of (111)In-DTPA-G4/PEI/γ-PGA was observed in the first draining popliteal LN when compared with that of (111)In-DTPA-G4/PEI. Moreover, (111)In-DTPA-G4/PEI/γ-PGA clearly visualized the sentinel LN with single photon emission computed tomography (SPECT) compared with (111)In-DTPA-G4/PEI. Thus, (111)In-DTPA-G4/PEI/γ-PGA can be useful as a nano-platform for molecular imaging including sentinel LN imaging.
|
| 巻・号 |
194
|
| ページ |
310-5
|
| 公開日 |
2014-11-28
|
| DOI |
10.1016/j.jconrel.2014.08.025
|
| PII |
S0168-3659(14)00612-9
|
| PMID |
25238958
|
| MeSH |
Animals
Erythrocytes / drug effects
Indium Radioisotopes* / chemistry
Indium Radioisotopes* / pharmacokinetics
Lymph Nodes / diagnostic imaging*
Macrophages / drug effects
Macrophages / metabolism
Mice
Nanoparticles*
Pentetic Acid / chemistry
Phagocytosis / drug effects
Polyglutamic Acid / analogs & derivatives*
Polyglutamic Acid / chemistry
Polyglutamic Acid / pharmacokinetics
Radiopharmaceuticals* / chemistry
Radiopharmaceuticals* / pharmacokinetics
Rats
Tissue Distribution
Tomography, Emission-Computed, Single-Photon
|
| IF |
7.727
|
| 引用数 |
5
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
CHEMISTRY, MULTIDISCIPLINARY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
