| RRC ID |
45875
|
| Author |
Wang P, Liu B, Zhang D, Belew MY, Tissenbaum HA, Cheng JX.
|
| Title |
Imaging lipid metabolism in live Caenorhabditis elegans using fingerprint vibrations.
|
| Journal |
Angew Chem Int Ed Engl
|
| Abstract |
Quantitation of lipid storage, unsaturation, and oxidation in live C. elegans has been a long-standing obstacle. The combination of hyperspectral stimulated Raman scattering imaging and multivariate analysis in the fingerprint vibration region represents a platform that allows the quantitative mapping of fat distribution, degree of fat unsaturation, lipid oxidation, and cholesterol storage in vivo in the whole worm. Our results reveal for the first time that lysosome-related organelles in intestinal cells are sites for storage of cholesterol in C. elegans.
|
| Volume |
53(44)
|
| Pages |
11787-92
|
| Published |
2014-10-27
|
| DOI |
10.1002/anie.201406029
|
| PMID |
25195517
|
| PMC |
PMC4348091
|
| MeSH |
Animals
Caenorhabditis elegans / chemistry*
Dermatoglyphics
Lipids / chemistry*
Metabolic Networks and Pathways*
Spectrum Analysis / methods*
Vibration
|
| IF |
12.959
|
| Times Cited |
45
|
|
WOS Category
|
CHEMISTRY, MULTIDISCIPLINARY
|
| Resource |
| C.elegans |
tm331
tm420 |
