論文 - 詳細
| RRC ID | 45902 |
|---|---|
| 著者 | Zheng Q, Ahlawat S, Schaefer A, Mahoney T, Koushika SP, Nonet ML. |
| タイトル | The vesicle protein SAM-4 regulates the processivity of synaptic vesicle transport. |
| ジャーナル | PLoS Genet |
| Abstract |
Axonal transport of synaptic vesicles (SVs) is a KIF1A/UNC-104 mediated process critical for synapse development and maintenance yet little is known of how SV transport is regulated. Using C. elegans as an in vivo model, we identified SAM-4 as a novel conserved vesicular component regulating SV transport. Processivity, but not velocity, of SV transport was reduced in sam-4 mutants. sam-4 displayed strong genetic interactions with mutations in the cargo binding but not the motor domain of unc-104. Gain-of-function mutations in the unc-104 motor domain, identified in this study, suppress the sam-4 defects by increasing processivity of the SV transport. Genetic analyses suggest that SAM-4, SYD-2/liprin-α and the KIF1A/UNC-104 motor function in the same pathway to regulate SV transport. Our data support a model in which the SV protein SAM-4 regulates the processivity of SV transport. |
| 巻・号 | 10(10) |
| ページ | e1004644 |
| 公開日 | 2014-10-1 |
| DOI | 10.1371/journal.pgen.1004644 |
| PII | PGENETICS-D-13-03398 |
| PMID | 25329901 |
| PMC | PMC4199485 |
| MeSH | Animals Animals, Genetically Modified Axonal Transport / genetics Binding Sites Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Intercellular Signaling Peptides and Proteins Membrane Proteins / genetics Membrane Proteins / metabolism* Mutation Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neurites / metabolism Neurons / metabolism Phosphoproteins / genetics Phosphoproteins / metabolism Synaptic Vesicles / metabolism* |
| IF | 5.175 |
| 引用数 | 20 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 線虫 | tm3828 |