論文 - 詳細
| RRC ID | 46001 |
|---|---|
| 著者 | Käser-Pébernard S, Müller F, Wicky C. |
| タイトル | LET-418/Mi2 and SPR-5/LSD1 cooperatively prevent somatic reprogramming of C. elegans germline stem cells. |
| ジャーナル | Stem Cell Reports |
| Abstract |
Throughout their journey to forming new individuals, germline stem cells must remain totipotent, particularly by maintaining a specific chromatin structure. However, the place epigenetic factors occupy in this process remains elusive. So far, "sensitization" of chromatin by modulation of histone arrangement and/or content was believed to facilitate transcription-factor-induced germ cell reprogramming. Here, we demonstrate that the combined reduction of two epigenetic factors suffices to reprogram C. elegans germ cells. The histone H3K4 demethylase SPR-5/LSD1 and the chromatin remodeler LET-418/Mi2 function together in an early process to maintain germ cell status and act as a barrier to block precocious differentiation. This epigenetic barrier is capable of limiting COMPASS-mediated H3K4 methylation, because elevated H3K4me3 levels correlate with germ cell reprogramming in spr-5; let-418 mutants. Interestingly, germ cells deficient for spr-5 and let-418 mainly reprogram as neurons, suggesting that neuronal fate might be the first to be derepressed in early embryogenesis. |
| 巻・号 | 2(4) |
| ページ | 547-59 |
| 公開日 | 2014-4-8 |
| DOI | 10.1016/j.stemcr.2014.02.007 |
| PII | S2213-6711(14)00059-9 |
| PMID | 24749077 |
| PMC | PMC3986580 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Differentiation / genetics Cell Division / genetics Cell Transformation, Neoplastic / genetics Cellular Reprogramming* Chromatin / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Down-Regulation Female Gene Expression Regulation Germ Cells / cytology Germ Cells / metabolism* Histones / metabolism Male Methylation Mi-2 Nucleosome Remodeling and Deacetylase Complex / metabolism* Mutation Neoplasms, Germ Cell and Embryonal / genetics Neurons / cytology Neurons / metabolism Oxidoreductases, N-Demethylating / genetics Oxidoreductases, N-Demethylating / metabolism* Protein Binding Stem Cells / cytology Stem Cells / metabolism* |
| IF | 6.032 |
| 引用数 | 19 |
| WOS 分野 | CELL & TISSUE ENGINEERING CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm1231 |