| RRC ID |
46829
|
| 著者 |
Wang DN, Toyotome T, Muraosa Y, Watanabe A, Wuren T, Bunsupa S, Aoyagi K, Yamazaki M, Takino M, Kamei K.
|
| タイトル |
GliA in Aspergillus fumigatus is required for its tolerance to gliotoxin and affects the amount of extracellular and intracellular gliotoxin.
|
| ジャーナル |
Med Mycol
|
| Abstract |
Gliotoxin is an important virulence factor of Aspergillus fumigatus. Although GliA putatively belongs to the major facilitator superfamily in the gliotoxin biosynthesis cluster, its roles remain unclear. To determine the function of GliA, we disrupted gliA in A. fumigatus. gliA disruption increased the susceptibility of A. fumigatus to gliotoxin. The gliT and gliA double-disrupted mutant had even higher susceptibility to gliotoxin than each individual disruptant. The extracellular release of gliotoxin was greatly decreased in the gliA disruptant. Mice infected with the gliA disruptant of A. fumigatus showed higher survival rates than those infected with the parent strain. These results strongly indicate that GliA, in addition to GliT, plays a significant role in the tolerance to gliotoxin and protection from extracellular gliotoxin in A. fumigatus by exporting the toxin. This also allows the fungus to evade the harmful effect of its own gliotoxin production. Moreover, GliA contributes to the virulence of A. fumigatus through gliotoxin secretion.
|
| 巻・号 |
52(5)
|
| ページ |
506-18
|
| 公開日 |
2014-7-1
|
| DOI |
10.1093/mmy/myu007
|
| PII |
myu007
|
| PMID |
24847038
|
| MeSH |
Amino Acid Sequence
Animals
Aspergillosis / microbiology*
Aspergillus fumigatus / drug effects
Aspergillus fumigatus / genetics*
Aspergillus fumigatus / pathogenicity
Aspergillus fumigatus / physiology
Cell Line
Fungal Proteins / genetics
Fungal Proteins / metabolism*
Gene Deletion
Gliotoxin / metabolism*
Humans
Immunocompromised Host
Male
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Multigene Family
Mutation
Oxidoreductases / genetics
Oxidoreductases / metabolism
Sequence Alignment
Virulence
Virulence Factors
|
| IF |
2.822
|
| 引用数 |
18
|
|
WOS 分野
|
VETERINARY SCIENCES
MYCOLOGY
INFECTIOUS DISEASES
|
| リソース情報 |
| 病原微生物 |
|
