論文 - 詳細
| RRC ID | 46939 |
|---|---|
| 著者 | Takizawa K, Hashizume T, Kamei K. |
| タイトル | Occurrence and characteristics of group 1 introns found at three different positions within the 28S ribosomal RNA gene of the dematiaceous Phialophora verrucosa: phylogenetic and secondary structural implications. |
| ジャーナル | BMC Microbiol |
| Abstract |
BACKGROUND:Group 1 introns (ribozymes) are among the most ancient and have the broadest phylogenetic distribution among the known self-splicing ribozymes. Fungi are known to be rich in rDNA group 1 introns. In the present study, five sequences of the 28S ribosomal RNA gene (rDNA) regions of pathogenic dematiaceous Phialophora verrucosa were analyzed using PCR by site-specific primers and were found to have three insertions, termed intron-F, G and H, at three positions of the gene. We investigated the distribution of group 1 introns in this fungus by surveying 34 strains of P. verrucosa and seven strains of Phialophora americana as the allied species. RESULTS:Intron-F's (inserted at L798 position) were found in 88% of P. verrucosa strains, while intron-G's (inserted at L1921) at 12% and intron-H's (inserted at L2563) at 18%. There was some correlation between intron distribution and geographic location. In addition, we confirmed that the three kinds of introns are group 1 introns from results of BLAST search, alignment analysis and Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Prediction of secondary structures and phylogenetic analysis of intron sequences identified introns-F and G as belonging to subgroup IC1. In addition, intron-H was identified as IE. CONCLUSION:The three intron insertions and their insertion position in the 28S rDNA allowed the characterization of the clinical and environmental isolates of P. verrucosa and P. americana into five genotypes. All subgroups of introns-F and G and intron-H were characterized and observed for the first time in both species. |
| 巻・号 | 11 |
| ページ | 94 |
| 公開日 | 2011-5-8 |
| DOI | 10.1186/1471-2180-11-94 |
| PII | 1471-2180-11-94 |
| PMID | 21548984 |
| PMC | PMC3112068 |
| MeSH | DNA Primers / genetics Environmental Microbiology Genotype Humans Introns* Molecular Sequence Data Mycological Typing Techniques Mycoses / microbiology Nucleic Acid Conformation* Phialophora / genetics* Phialophora / isolation & purification Phylogeny* Polymerase Chain Reaction / methods RNA, Ribosomal, 28S / genetics* Sequence Analysis, DNA Sequence Homology, Nucleic Acid |
| IF | 2.989 |
| 引用数 | 5 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 病原微生物 | IFM 4928 IFM 5089 IFM 41710 IFM 41721 IFM 41724 IFM 41725 IFM 41739 IFM 41740 IFM 41746 IFM 41749 IFM 41750 IFM 41752 IFM 41755 IFM 41764 IFM 41765 IFM 41778 IFM 41779 IFM 41780 IFM 41871 IFM 41872 IFM 41879 IFM 41881 IFM 41883 IFM 41884 IFM 41885 IFM 41886 IFM 41887 IFM 41888 IFM 41892 IFM 41893 IFM 41897 IFM 41898 IFM 41899 IFM 51934 |