RRC ID |
47502
|
著者 |
Iwasaki T, Kappl R, Bracic G, Shimizu N, Ohmori D, Kumasaka T.
|
タイトル |
ISC-like [2Fe-2S] ferredoxin (FdxB) dimer from Pseudomonas putida JCM 20004: structural and electron-nuclear double resonance characterization.
|
ジャーナル |
J Biol Inorg Chem
|
Abstract |
The crystal structure of the ISC-like [2Fe-2S] ferredoxin (FdxB), probably involved in the de novo iron-sulfur cluster biosynthesis (ISC) system of Pseudomonas putida JCM 20004, was determined at 1.90-Å resolution and displayed a novel tail-to-tail dimeric form. P. putida FdxB lacks the consensus free cysteine usually present near the cluster of ISC-like ferredoxins, indicating its primarily electron transfer role in the iron-sulfur cluster. Orientation-selective electron-nuclear double resonance spectroscopic analysis of reduced FdxB in conjunction with the crystal structure has identified the innermost Fe2 site with a high positive spin population as the nonreducible iron retaining the Fe(3+) valence and the outermost Fe1 site as the reduced iron with a low negative spin density. The average g (max) direction is skewed, forming an angle of about 27.3° (±4°) with the normal of the [2Fe-2S] plane, whereas the g (int) and g (min) directions are distributed in the cluster plane, presumably tilted by the same angle with respect to this plane. These results are related to those for other [2Fe-2S] proteins in different electron transport chains (e.g. adrenodoxin) and suggest a significant distortion of the electronic structure of the reduced [2Fe-2S] cluster under the influence of the protein environment around each iron site in general.
|
巻・号 |
16(6)
|
ページ |
923-35
|
公開日 |
2011-8-1
|
DOI |
10.1007/s00775-011-0793-8
|
PMID |
21647778
|
MeSH |
Amino Acid Sequence
Bacterial Proteins / chemistry*
Bacterial Proteins / metabolism
Crystallography, X-Ray
Electron Spin Resonance Spectroscopy / methods*
Ferredoxins / chemistry*
Ferredoxins / metabolism
Models, Molecular
Molecular Sequence Data
Protein Conformation
Protein Multimerization
Pseudomonas putida / chemistry
Pseudomonas putida / metabolism*
Sequence Alignment
|
IF |
3.246
|
引用数 |
9
|
WOS 分野
|
CHEMISTRY, INORGANIC & NUCLEAR
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
一般微生物 |
JCM 20004 |