| RRC ID |
47541
|
| 著者 |
Cai J, Xie Y, Song B, Wang Y, Zhang Z, Feng Y.
|
| タイトル |
Fervidobacterium changbaicum Lip1: identification, cloning, and characterization of the thermophilic lipase as a new member of bacterial lipase family V.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
A novel lipase gene encoded 315 amino acid residues was obtained using lipase-prospecting primers and genome walking from hyperthermophilic bacterium Fervidobacterium changbaicum CBS-1. Sequence alignment and phylogenetic analysis revealed this novel lipase is a new member of bacterial lipase family V. The recombinant enzyme F. changbaicum lipase 1 (FCLip1) showed maximum activity at 78 °C and pH 7.8. It displayed extreme thermostability at 70 °C and was also stable across a wide pH range from 6.0 to 12.0. Kinetic study demonstrated FCLip1 preferentially hydrolyzed middle-length acyl chains, especially p-nitrophenyl caprate and tricaprylin. With p-nitrophenyl caprate as a substrate, the enzyme exhibited a K(m) and k(cat) of 4.67 μM and 22.7/s, respectively. In addition, FCLip1 was resistant to various detergents and organic solvents. This enzyme is the first reported thermophilic lipase from bacterial family Thermotogaceae. Its extreme stability with respect to temperature and pH, along with its triglyceride hydrolysis activity, indicate that FCLip1 has high potential for future application.
|
| 巻・号 |
89(5)
|
| ページ |
1463-73
|
| 公開日 |
2011-3-1
|
| DOI |
10.1007/s00253-010-2971-y
|
| PMID |
21046373
|
| MeSH |
Amino Acid Sequence
Bacteria / enzymology*
Bacteria / genetics
Cloning, Molecular
Enzyme Stability
Hot Temperature
Hydrogen-Ion Concentration
Kinetics
Lipase / chemistry
Lipase / genetics*
Lipase / metabolism*
Molecular Sequence Data
Phylogeny
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Substrate Specificity
|
| IF |
3.53
|
| 引用数 |
11
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 13353 |
