RRC ID 47666
Author Savitsky M, Kim M, Kravchuk O, Schwartz YB.
Title Distinct Roles of Chromatin Insulator Proteins in Control of the Drosophila Bithorax Complex.
Journal Genetics
Abstract Chromatin insulators are remarkable regulatory elements that can bring distant genomic sites together and block unscheduled enhancer-promoter communications. Insulators act via associated insulator proteins of two classes: sequence-specific DNA binding factors and "bridging" proteins. The latter are required to mediate interactions between distant insulator elements. Chromatin insulators are critical for correct expression of complex loci; however, their mode of action is poorly understood. Here, we use the Drosophila bithorax complex as a model to investigate the roles of the bridging proteins Cp190 and Mod(mdg4). The bithorax complex consists of three evolutionarily conserved homeotic genes Ubx, abd-A, and Abd-B, which specify anterior-posterior identity of the last thoracic and all abdominal segments of the fly. Looking at effects of CTCF, mod(mdg4), and Cp190 mutations on expression of the bithorax complex genes, we provide the first functional evidence that Mod(mdg4) acts in concert with the DNA binding insulator protein CTCF. We find that Mod(mdg4) and Cp190 are not redundant and may have distinct functional properties. We, for the first time, demonstrate that Cp190 is critical for correct regulation of the bithorax complex and show that Cp190 is required at an exceptionally strong Fub insulator to partition the bithorax complex into two topological domains.
Volume 202(2)
Pages 601-17
Published 2016-2-1
DOI 10.1534/genetics.115.179309
PII genetics.115.179309
PMID 26715665
PMC PMC4788238
MeSH Alleles Animals Chromatin / genetics* Chromatin / metabolism* Drosophila / embryology Drosophila / genetics* Drosophila / metabolism* Drosophila Proteins / genetics* Drosophila Proteins / metabolism* Epistasis, Genetic Gene Expression Regulation Gene Order Genes, Homeobox Insulator Elements* Mutation Organ Specificity / genetics Phenotype Protein Binding
IF 4.015
Times Cited 9
WOS Category GENETICS & HEREDITY
Resource
Drosophila DGRC#204647