論文 - 詳細
RRC ID | 4806 |
---|---|
著者 | Dedeurwaerder S, Menu-Bouaouiche L, Mareck A, Lerouge P, Guerineau F. |
タイトル | Activity of an atypical Arabidopsis thaliana pectin methylesterase. |
ジャーナル | Planta |
Abstract |
An Arabidopsis thaliana pectin methylesterase that was not predicted to contain any signaling sequence was produced in E. coli and purified using a His tag added at its N-terminus. The enzyme demethylesterified Citrus pectin with a Km of 0.86 mg/ml. The enzyme did not require salt for activity and was found to be relatively temperature-sensitive. The precipitation of enzyme-treated pectin by CaCl2 suggested that the enzyme had a blockwise mode of pectin demethylesterification. A purified kiwi (Actinidia chinensis) pectin methylesterase inhibitor had no effect on the activity of the enzyme whereas it strongly inhibited a flax pectin methylesterase. A model of the protein structure revealed that an extra amino acid sequence in this particular Arabidopsis pectin methylesterase could form a ss-strand outside the core structure, which might be preventing the inhibitor from binding the protein. |
巻・号 | 229(2) |
ページ | 311-21 |
公開日 | 2009-1-1 |
DOI | 10.1007/s00425-008-0831-0 |
PMID | 18936961 |
MeSH | Actinidia / enzymology Amino Acid Sequence Arabidopsis / drug effects Arabidopsis / enzymology* Arabidopsis Proteins / antagonists & inhibitors Arabidopsis Proteins / chemistry Arabidopsis Proteins / isolation & purification Arabidopsis Proteins / metabolism* Carboxylic Ester Hydrolases / antagonists & inhibitors Carboxylic Ester Hydrolases / chemistry Carboxylic Ester Hydrolases / isolation & purification Carboxylic Ester Hydrolases / metabolism* Catalytic Domain Electrophoresis, Polyacrylamide Gel Enzyme Inhibitors / pharmacology Models, Molecular Molecular Sequence Data Protein Structure, Secondary Sequence Alignment Sequence Analysis, Protein |
IF | 3.39 |
引用数 | 17 |
WOS 分野 | PLANT SCIENCES |
リソース情報 | |
シロイヌナズナ / 植物培養細胞・遺伝子 | pda06543 |