論文 - 詳細
RRC ID | 4972 |
---|---|
著者 | Liu IF, Annamalai T, Sutherland JH, Tse-Dinh YC. |
タイトル | Hydroxyl radicals are involved in cell killing by the bacterial topoisomerase I cleavage complex. |
ジャーナル | J Bacteriol |
Abstract |
Escherichia coli expressing SOS-inducing mutant topoisomerase I was utilized to demonstrate that covalent protein-DNA complex accumulation results in oxidative damage. Hydroxyl radicals were detected following mutant topoisomerase induction. The presence of the Fe(2+) chelator 2,2'-dipyridyl and an iscS mutation affecting Fe-S cluster formation protect against topoisomerase I cleavage complex-mediated cell killing. |
巻・号 | 191(16) |
ページ | 5315-9 |
公開日 | 2009-8-1 |
DOI | 10.1128/JB.00559-09 |
PII | JB.00559-09 |
PMID | 19525344 |
PMC | PMC2725605 |
MeSH | 2,2'-Dipyridyl / pharmacology Anti-Bacterial Agents / pharmacology Arabinose / pharmacology DNA / metabolism DNA Topoisomerases, Type I / genetics DNA Topoisomerases, Type I / metabolism DNA Topoisomerases, Type I / physiology* Escherichia coli / drug effects Escherichia coli / genetics Escherichia coli / metabolism* Hydroxyl Radical / metabolism* Iron Chelating Agents / pharmacology Mutation Norfloxacin / pharmacology |
IF | 3.006 |
引用数 | 18 |
WOS 分野 | MICROBIOLOGY |
リソース情報 | |
原核生物(大腸菌) | Keio collection JW2514 |