| RRC ID |
49967
|
| 著者 |
Yamamoto K, Kawai-Toyooka H, Hamaji T, Tsuchikane Y, Mori T, Takahashi F, Sekimoto H, Ferris PJ, Nozaki H.
|
| タイトル |
Molecular evolutionary analysis of a gender-limited MID ortholog from the homothallic species Volvox africanus with male and monoecious spheroids.
|
| ジャーナル |
PLoS One
|
| Abstract |
Volvox is a very interesting oogamous organism that exhibits various types of sexuality and/or sexual spheroids depending upon species or strains. However, molecular bases of such sexual reproduction characteristics have not been studied in this genus. In the model species V. carteri, an ortholog of the minus mating type-determining or minus dominance gene (MID) of isogamous Chlamydomonas reinhardtii is male-specific and determines the sperm formation. Male and female genders are genetically determined (heterothallism) in V. carteri, whereas in several other species of Volvox both male and female gametes (sperm and eggs) are formed within the same clonal culture (homothallism). To resolve the molecular basis of the evolution of Volvox species with monoecious spheroids, we here describe a MID ortholog in the homothallic species V. africanus that produces both monoecious and male spheroids within a single clonal culture. Comparison of synonymous and nonsynonymous nucleotide substitutions in MID genes between V. africanus and heterothallic volvocacean species suggests that the MID gene of V. africanus evolved under the same degree of functional constraint as those of the heterothallic species. Based on semi quantitative reverse transcription polymerase chain reaction analyses using the asexual, male and monoecious spheroids isolated from a sexually induced V. africanus culture, the MID mRNA level was significantly upregulated in the male spheroids, but suppressed in the monoecious spheroids. These results suggest that the monoecious spheroid-specific down regulation of gene expression of the MID homolog correlates with the formation of both eggs and sperm in the same spheroid in V. africanus.
|
| 巻・号 |
12(6)
|
| ページ |
e0180313
|
| 公開日 |
2017-6-30
|
| DOI |
10.1371/journal.pone.0180313
|
| PII |
PONE-D-17-12057
|
| PMID |
28665990
|
| PMC |
PMC5493378
|
| MeSH |
Blotting, Southern
Evolution, Molecular*
Genes, Plant*
Ovule
Phylogeny
Pollen*
Polymerase Chain Reaction
Reproduction
Reverse Transcriptase Polymerase Chain Reaction
Species Specificity
Spheroids, Cellular*
Volvox / classification
Volvox / genetics*
Volvox / physiology
|
| IF |
2.74
|
| 引用数 |
6
|
| リソース情報 |
| 藻類 |
NIES-3780
NIES-3986
NIES-3786
NIES-3782
NIES-3783
NIES-3785 |
