論文 - 詳細
RRC ID | 50620 |
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著者 | Sugimoto R, Saito N, Shimada T, Tanaka K. |
タイトル | Identification of YbhA as the pyridoxal 5'-phosphate (PLP) phosphatase in Escherichia coli: Importance of PLP homeostasis on the bacterial growth. |
ジャーナル | J Gen Appl Microbiol |
Abstract |
The gene ybhA of Escherichia coli encodes a phosphatase that has an in vitro specificity to dephosphorylate pyridoxal 5'-phosphate (PLP or vitamin B6), a co-factor for aminotransferases and other enzymes. In this study, we found that excess pyridoxal (PL) in a minimal medium resulted in excess PLP in vivo and growth inhibition, which was alleviated by YbhA overproduction. Conversely, the YbhA overproduction resulted in PLP shortage in vivo and the correlated reduction in growth rate, which was significantly negated by PL in the medium. In addition, the overproduction of a PL kinase, PdxK or PdxY, was inhibitory to cell growth only in the absence of the functional ybhA gene, and the growth defects were alleviated by casamino acids in the medium, which suggested that both the shortage of, and excess, PLP resulted in the disturbance of amino acid metabolism and cell growth, as revealed by a metabolome analysis. |
巻・号 | 63(6) |
ページ | 362-368 |
公開日 | 2018-1-15 |
DOI | 10.2323/jgam.2017.02.008 |
PMID | 29187681 |
MeSH | Amino Acids / metabolism Escherichia coli / enzymology* Escherichia coli / genetics* Escherichia coli / growth & development Escherichia coli / metabolism Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Homeostasis / genetics* Metabolome Phosphoric Monoester Hydrolases / genetics Phosphoric Monoester Hydrolases / metabolism* Pyridoxal / metabolism Pyridoxal Phosphate / deficiency Pyridoxal Phosphate / genetics Pyridoxal Phosphate / metabolism* Vitamin B 6 / metabolism |
IF | 0.789 |
引用数 | 5 |
リソース情報 | |
原核生物(大腸菌) | JW0749 |