| RRC ID |
50830
|
| 著者 |
Besse A, Vandervennet M, Goulard C, Peduzzi J, Isaac S, Rebuffat S, Carré-Mlouka A.
|
| タイトル |
Halocin C8: an antimicrobial peptide distributed among four halophilic archaeal genera: Natrinema, Haloterrigena, Haloferax, and Halobacterium.
|
| ジャーナル |
Extremophiles
|
| Abstract |
Halophilic archaea thrive in hypersaline ecosystems and produce antimicrobial peptides (AMPs) named halocins. AMPs are essential effectors of microbial interactions in natural ecosystems. Halocin C8 is a 7.4 kDa peptide produced by Natrinema sp. AS7092. Surrounded by genes involved in regulation and transport, the halC8 gene encodes a precursor, processed into the mature halocin and an immunity protein, protecting the producing strain against its halocin. This feature constitutes a unique property of halocin C8, as known AMPs and their immunity proteins are generally encoded on distinct ORFs in an operon. By complementary in silico and PCR-based approaches, the presence of halC8 in halophilic archaea collected from various parts of the world was evidenced. The full-length halC8 gene is restricted and consistently found in the genomes of strains belonging to the phylogenetically related genera Natrinema and Haloterrigena, along with transport and regulation genes. Functional expression of halC8 was demonstrated by RT-PCR and antimicrobial assays. Active halocin C8 was shown to contain five disulphide bridges, presumably conferring a compact structure resistant to harsh environmental conditions. In other archaeal genera, Haloferax and Halobacterium, genes encoding halocin C8 with diverging immunity protein moiety were evidenced. A phylogenetic analysis of halocin C8 sequences was conducted.
|
| 巻・号 |
21(3)
|
| ページ |
623-638
|
| 公開日 |
2017-5-1
|
| DOI |
10.1007/s00792-017-0931-5
|
| PII |
10.1007/s00792-017-0931-5
|
| PMID |
28386790
|
| MeSH |
Archaeal Proteins / chemistry
Archaeal Proteins / genetics*
Archaeal Proteins / metabolism
Bacteriocins / chemistry
Bacteriocins / genetics*
Bacteriocins / metabolism
Disulfides / chemistry
Extreme Environments
Genes, Archaeal
Halobacteriaceae / classification
Halobacteriaceae / genetics*
Halobacteriaceae / metabolism
Open Reading Frames
Operon
Phylogeny
Salinity
|
| IF |
2.462
|
| 引用数 |
4
|
| リソース情報 |
| 一般微生物 |
JCM 12980
JCM 13891
JCM 12890
JCM 14663
JCM 15624
JCM 13563 |
