RRC ID |
50956
|
著者 |
Gotoh A, Nara M, Sugiyama Y, Sakanaka M, Yachi H, Kitakata A, Nakagawa A, Minami H, Okuda S, Katoh T, Katayama T, Kurihara S.
|
タイトル |
Use of Gifu Anaerobic Medium for culturing 32 dominant species of human gut microbes and its evaluation based on short-chain fatty acids fermentation profiles.
|
ジャーナル |
Biosci Biotechnol Biochem
|
Abstract |
Recently, a "human gut microbial gene catalogue," which ranks the dominance of microbe genus/species in human fecal samples, was published. Most of the bacteria ranked in the catalog are currently publicly available; however, the growth media recommended by the distributors vary among species, hampering physiological comparisons among the bacteria. To address this problem, we evaluated Gifu anaerobic medium (GAM) as a standard medium. Forty-four publicly available species of the top 56 species listed in the "human gut microbial gene catalogue" were cultured in GAM, and out of these, 32 (72%) were successfully cultured. Short-chain fatty acids from the bacterial culture supernatants were then quantified, and bacterial metabolic pathways were predicted based on in silico genomic sequence analysis. Our system provides a useful platform for assessing growth properties and analyzing metabolites of dominant human gut bacteria grown in GAM and supplemented with compounds of interest.
|
巻・号 |
81(10)
|
ページ |
2009-2017
|
公開日 |
2017-10-1
|
DOI |
10.1080/09168451.2017.1359486
|
PMID |
28782454
|
MeSH |
Anaerobiosis
Bacteria / genetics
Bacteria / growth & development*
Bacteria / metabolism*
Computer Simulation
Culture Techniques
DNA, Bacterial / genetics
Fatty Acids, Volatile / metabolism*
Fermentation*
Gastrointestinal Microbiome*
Genomics
|
IF |
1.516
|
引用数 |
8
|
リソース情報 |
一般微生物 |
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