RRC ID |
51549
|
著者 |
Ahier A, Dai CY, Tweedie A, Bezawork-Geleta A, Kirmes I, Zuryn S.
|
タイトル |
Affinity purification of cell-specific mitochondria from whole animals resolves patterns of genetic mosaicism.
|
ジャーナル |
Nat Cell Biol
|
Abstract |
Although mitochondria are ubiquitous organelles, they exhibit tissue-specific morphology, dynamics and function. Here, we describe a robust approach to isolate mitochondria from specific cells of diverse tissue systems in Caenorhabditis elegans. Cell-specific mitochondrial affinity purification (CS-MAP) yields intact and functional mitochondria with exceptional purity and sensitivity (>96% enrichment, >96% purity, and single-cell and single-animal resolution), enabling comparative analyses of protein and nucleic acid composition between organelles isolated from distinct cellular lineages. In animals harbouring a mixture of mutant and wild-type mitochondrial genomes, we use CS-MAP to reveal subtle mosaic patterns of cell-type-specific heteroplasmy across large populations of animals (>10,000 individuals). We demonstrate that the germline is more prone to propagating deleterious mitochondrial genomes than somatic lineages, which we propose is caused by enhanced mtDNA replication in this tissue.
|
巻・号 |
20(3)
|
ページ |
352-360
|
公開日 |
2018-3-1
|
DOI |
10.1038/s41556-017-0023-x
|
PII |
10.1038/s41556-017-0023-x
|
PMID |
29358705
|
MeSH |
Animals
Animals, Genetically Modified
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / metabolism
Cell Fractionation / methods*
Chromatography, Affinity*
DNA Replication
DNA, Mitochondrial / biosynthesis
DNA, Mitochondrial / genetics*
Microscopy, Confocal
Mitochondria / genetics*
Mitochondria / metabolism
Mosaicism*
Mutation
Organ Specificity
|
IF |
20.042
|
引用数 |
16
|
リソース情報 |
線虫 |
tm1779 |