論文 - 詳細
| RRC ID | 51597 |
|---|---|
| 著者 | Ogawa K, Miyaji H, Kato A, Kosen Y, Momose T, Yoshida T, Nishida E, Miyata S, Murakami S, Takita H, Fugetsu B, Sugaya T, Kawanami M. |
| タイトル | Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs. |
| ジャーナル | J Periodontal Res |
| Abstract |
BACKGROUND AND OBJECTIVE:Nanoparticle bioceramics are being investigated for biomedical applications. We fabricated a regenerative scaffold comprising type I collagen and beta-tricalcium phosphate (β-TCP) nanoparticles. Fibroblast growth factor-2 (FGF-2) is a bioeffective signaling molecule that stimulates cell proliferation and wound healing. This study examined the effects, on bioactivity, of a nano-β-TCP/collagen scaffold loaded with FGF-2, particularly on periodontal tissue wound healing. MATERIAL AND METHODS:Beta-tricalcium phosphate was pulverized into nanosize particles (84 nm) and was then dispersed. A nano-β-TCP scaffold was prepared by coating the surface of a collagen scaffold with a nanosize β-TCP dispersion. Scaffolds were characterized using scanning electron microscopy, compressive testing, cell seeding and rat subcutaneous implant testing. Then, nano-β-TCP scaffold, nano-β-TCP scaffold loaded with FGF-2 and noncoated collagen scaffold were implanted into a dog one-wall infrabony defect model. Histological observations were made at 10 d and 4 wk postsurgery. RESULTS:Scanning electron microscopy images show that TCP nanoparticles were attached to collagen fibers. The nano-β-TCP scaffold showed higher compressive strength and cytocompatibility compared with the noncoated collagen scaffold. Rat subcutaneous implant tests showed that the DNA contents of infiltrating cells in the nano-β-TCP scaffold and the FGF-2-loaded scaffold were approximately 2.8-fold and 3.7-fold greater, respectively, than in the collagen scaffold. Histological samples from the periodontal defect model showed about five-fold greater periodontal tissue repair following implantation of the nano-β-TCP scaffold loaded with FGF-2 compared with the collagen scaffold. CONCLUSION:The β-TCP nanoparticle coating strongly improved the collagen scaffold bioactivity. Nano-β-TCP scaffolds containing FGF-2 are anticipated for use in periodontal tissue engineering. |
| 巻・号 | 51(6) |
| ページ | 758-767 |
| 公開日 | 2016-12-1 |
| DOI | 10.1111/jre.12352 |
| PMID | 27870141 |
| MeSH | Animals Biocompatible Materials / therapeutic use Calcium Phosphates / therapeutic use* Collagen Type I / therapeutic use Dogs Female Fibroblast Growth Factor 2 / therapeutic use* Male Microscopy, Electron, Scanning Nanoparticles / therapeutic use* Periodontium / growth & development* Periodontium / ultrastructure Rats Rats, Wistar Tissue Engineering / methods* Tissue Scaffolds* Wound Healing |
| IF | 2.878 |
| 引用数 | 21 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |