Reference - Detail
RRC ID | 5162 |
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Author | Uchiyama H, Hashidoko Y, Kuriyama Y, Tahara S. |
Title | Identification of the 4-hydroxycinnamate decarboxylase (PAD) gene of Klebsiella oxytoca. |
Journal | Biosci Biotechnol Biochem |
Abstract |
A 7.1-kbp DNA fragment isolated from a wild strain of Klebsiella oxytoca was sequenced, leading to the identification of 10 open-reading frames (ORFs), including a 504-bp Pad gene. The Pad gene of the Gram-negative bacterium was subsequently expressed in Escherichia coli as a chimeric Pad. The deduced amino acid (AA) sequence of the Pad gene from wild-type K. oxytoca showed approximately 50% homology to those of other bacterial PADs from Gram-positive bacilli plus a coccus. These data and a genomic library search of some gamma-proteobacteria, including E. coli and Vibrio sp., indicated that PAD of K. oxytoca is a member of the bacterial PAD family characteristic of Gram-negative bacteria. Using Pad-specific PCR primers designed from the Gram-negative bacterial Pad of K. oxytoca, Pad genes of two further strains of K. oxytoca, another wild isolate and JCM 1665 and two PAD-positive Enterobacter spp. were successfully amplified for specific Pad detection. |
Volume | 72(1) |
Pages | 116-23 |
Published | 2008-1-1 |
DOI | 10.1271/bbb.70496 |
PII | JST.JSTAGE/bbb/70496 |
PMID | 18175898 |
MeSH | Amino Acid Sequence Bacterial Proteins / genetics Carboxy-Lyases / genetics* Conserved Sequence DNA Primers Gram-Negative Bacteria / enzymology Gram-Negative Bacteria / genetics Gram-Positive Bacteria / enzymology Gram-Positive Bacteria / genetics Klebsiella oxytoca / enzymology* Klebsiella oxytoca / genetics* Molecular Sequence Data Open Reading Frames Polymerase Chain Reaction Sequence Alignment Sequence Homology, Amino Acid Species Specificity |
IF | 1.516 |
Times Cited | 2 |
WOS Category | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
General Microbes | JCM1665 |