論文 - 詳細
| RRC ID | 51637 |
|---|---|
| 著者 | Yoo HY, Song YH, Foo M, Seo E, Hwang DS, Seo JH. |
| タイトル | Recombinant mussel proximal thread matrix protein promotes osteoblast cell adhesion and proliferation. |
| ジャーナル | BMC Biotechnol |
| Abstract |
BACKGROUND:von Willebrand factor (VWF) is a key load bearing domain for mamalian cell adhesion by binding various macromolecular ligands in extracellular matrix such as, collagens, elastin, and glycosaminoglycans. Interestingly, vWF like domains are also commonly found in load bearing systems of marine organisms such as in underwater adhesive of mussel and sea star, and nacre of marine abalone, and play a critical load bearing function. Recently, Proximal Thread Matrix Protein1 (PTMP1) in mussel composed of two vWF type A like domains has characterized and it is known to bind both mussel collagens and mammalian collagens. RESULTS:Here, we cloned and mass produced a recombinant PTMP1 from E. coli system after switching all the minor codons to the major codons of E. coli. Recombinant PTMP1 has an ability to enhance mouse osteoblast cell adhesion, spreading, and cell proliferation. In addition, PTMP1 showed vWF-like properties as promoting collagen expression as well as binding to collagen type I, subsequently enhanced cell viability. Consequently, we found that recombinant PTMP1 acts as a vWF domain by mediating cell adhesion, spreading, proliferation, and formation of actin cytoskeleton. CONCLUSIONS:This study suggests that both mammalian cell adhesion and marine underwater adhesion exploits a strong vWF-collagen interaction for successful wet adhesion. In addition, vWF like domains containing proteins including PTMP1 have a great potential for tissue engineering and the development of biomedical adhesives as a component for extra-cellular matrix. |
| 巻・号 | 16 |
| ページ | 16 |
| 公開日 | 2016-2-16 |
| DOI | 10.1186/s12896-016-0247-z |
| PII | 10.1186/s12896-016-0247-z |
| PMID | 26879700 |
| PMC | PMC4754843 |
| MeSH | Animals Bivalvia / genetics* Cell Adhesion / drug effects* Cell Line Cell Proliferation / drug effects* Cell Survival Collagen Escherichia coli / genetics Mice Protein Structure, Tertiary / genetics Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Recombinant Proteins / pharmacology* von Willebrand Factor / genetics |
| IF | 2.312 |
| 引用数 | 11 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |