論文 - 詳細
| RRC ID | 51704 |
|---|---|
| 著者 | Kumasaka A, Matsumoto N, Mukae S, Kitano T, Noguchi H, Ohki H, Komiyama K, Ando T. |
| タイトル | Rapid and Specific Screening Assay for KRAS Oncogene Mutation by a Novel Gene Amplification Method. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:Epidermal growth factor receptor (EGFR) is a target of molecular therapeutics for colorectal cancer. However, mutation of Kirsten rat sarcoma viral oncogene homolog (KRAS) gene at codons 12 and 13 attenuates the therapeutic effect of anti-EGFR therapies. Therefore, the detection of KRAS gene mutation is important for therapeutic decision-making. MATERIALS AND METHODS:KRAS gene mutation at codons 12 (c.34G>T, c.35G>C, c.35G>A) and 13 (c.38G>A) in six cancer cell lines were investigated. A loop-mediated isothermal amplification-based procedure was developed that employed peptide nucleic acid to suppress amplification of the wild-type allele. RESULTS:This mutation-oriented gene-amplification procedure can amplify the DNA fragment of the KRAS gene with codon 12 and codon 13 mutation within 30 min. Moreover, boiled cells can work as template resources. CONCLUSION:This newly developed procedure can be useful for patient stratification for anti-EGFR therapies. |
| 巻・号 | 36(4) |
| ページ | 1571-9 |
| 公開日 | 2016-4-1 |
| PII | 36/4/1571 |
| PMID | 27069133 |
| MeSH | Cell Line, Tumor Cells, Cultured DNA / genetics Gene Amplification Genes, ras / genetics* Humans Mouth Mucosa / cytology Mutation Nucleic Acid Amplification Techniques* |
| IF | 1.994 |
| 引用数 | 3 |
| リソース情報 | |
| ヒト・動物細胞 | HSC-3(RCB1975) |