論文 - 詳細
| RRC ID | 51707 |
|---|---|
| 著者 | Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Ishige N. |
| タイトル | Improved Survival and Initiation of Differentiation of Human Induced Pluripotent Stem Cells to Hepatocyte-Like Cells upon Culture in William's E Medium followed by Hepatocyte Differentiation Inducer Treatment. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:Hepatocyte differentiation inducer (HDI) lacks both glucose and arginine, but is supplemented with galactose and ornithine, and is added together with other reagents such as apoptosis inhibitor and oncostatin M. Although human induced pluripotent stem (iPS) cells initiate hepatocyte differentiation, most die within 7 days. In this study, we investigated both HDI and conventional media for their potential to improve cell survival. MATERIALS AND METHODS:201B7 iPS cells were cultured in conventional media. This consisted of three cycles of 5-day culture in William's E (WE) medium, followed by a 2-day culture in HDI. RESULTS:Expression levels of α-feto protein (AFP) were higher in cells cultured in WE and in Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham (DF12). 201B7 cells expressed the highest AFP and albumin (ALB) when cultured in HDI for 2 days following 7-day culture in WE. After three cycles of 5-day culture in WE followed by 2 days in HDI, 201B7 cells expressed AFP and ALB 54 ± 2.3 (average ± standard deviation) and 73 ± 15.1 times higher, respectively, than those cultured in ReproFF (feeder-free condition). CONCLUSION:201B7 cells survived culture in WE for 7 days followed HDI for 2 days. After three cycles of culture under these conditions, hepatocyte differentiation was enhanced, as evidenced by increased AFP and ALB expression. |
| 巻・号 | 11(4) |
| ページ | e0153435 |
| 公開日 | 2016-1-1 |
| DOI | 10.1371/journal.pone.0153435 |
| PII | PONE-D-15-44794 |
| PMID | 27073925 |
| PMC | PMC4830564 |
| MeSH | Cell Differentiation / drug effects* Cell Survival / drug effects* Cells, Cultured Culture Media Hepatocytes / cytology Hepatocytes / drug effects* Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / drug effects* Oncostatin M / pharmacology Organ Preservation Solutions alpha-Fetoproteins / metabolism |
| IF | 2.74 |
| 引用数 | 2 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |