| RRC ID |
51807
|
| 著者 |
Nakayama Y, Matsui S, Noda K, Yamazaki M, Iwai Y, Matsumura H, Izawa T, Tanaka E, Ganss B, Ogata Y.
|
| タイトル |
Amelotin gene expression is temporarily being upregulated at the initiation of apoptosis induced by TGFβ1 in mouse gingival epithelial cells.
|
| ジャーナル |
Apoptosis
|
| Abstract |
Amelotin (AMTN) is expressed and secreted by ameloblasts in the maturation stage of amelogenesis and persist with low levels in the junctional epithelium (JE) of erupted teeth. The purpose of this study is to investigate the transcriptional regulation of the AMTN gene by transforming growth factor beta1 (TGFβ1) in gingival epithelial (GE1) cells in the apoptosis phase. Apoptosis was evaluated by the fragmentation of chromosomal DNA and TUNEL staining. A real-time PCR was carried out to examine the AMTN mRNA levels induced by TGFβ1 and Smad3 overexpression. Transient transfection analyses were completed using the various lengths of mouse AMTN gene promoter constructs with or without TGFβ1. Chromatin immunoprecipitation (ChIP) assays were performed to investigate the Smad3 bindings to the AMTN gene promoter by TGFβ1. TGFβ1-induced apoptosis in GE1 cells were detected at 24 and 48 h by DNA fragmentation and TUNEL staining. AMTN mRNA levels increased at 6 h and reached maximum at 24 h in GE1 cells. Luciferase activities of the mouse AMTN gene promoter constructs were induced by TGFβ1. The results of the ChIP assays showed that there was an increase in Smad3 binding to Smad-binding element (SBE)#1 and SBE#2 after stimulation by TGFβ1. Immunohistochemical localization of AMTN was detected in the JE, and the AMTN protein levels in Smad3-deficient mice were decreased compared with wild-type mice. AMTN mRNA levels were induced at the initiation of apoptosis by TGFβ1, which mediated through the Smad3 bindings to SBEs in the mouse AMTN gene promoter.
|
| 巻・号 |
21(10)
|
| ページ |
1057-70
|
| 公開日 |
2016-10-1
|
| DOI |
10.1007/s10495-016-1279-5
|
| PII |
10.1007/s10495-016-1279-5
|
| PMID |
27502207
|
| MeSH |
Animals
Apoptosis*
Dental Enamel Proteins / genetics*
Dental Enamel Proteins / metabolism
Epithelial Cells / cytology
Epithelial Cells / metabolism*
Gingiva / cytology*
Gingiva / metabolism
Mice
Promoter Regions, Genetic
Smad3 Protein / genetics
Smad3 Protein / metabolism
Transforming Growth Factor beta1 / genetics*
Transforming Growth Factor beta1 / metabolism
Up-Regulation
|
| IF |
4.543
|
| 引用数 |
9
|
| リソース情報 |
| ヒト・動物細胞 |
GE1(RCB1709) |
