論文 - 詳細
RRC ID | 52627 |
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著者 | Yamada N, Kato-Kogoe N, Yamanegi K, Nishiura H, Fujihara Y, Fukunishi S, Okamura H, Terada N, Nakasho K. |
タイトル | Inhibition of Asparagine-linked Glycosylation Participates in Hypoxia-induced Down-regulation of Cell-surface MICA Expression. |
ジャーナル | Anticancer Res |
Abstract |
BACKGROUND/AIM:Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding. Recently, the inhibition of N-linked glycosylation was also shown to reduce the cell-surface expression of MICA. We investigated the participation of asparagine (Asn)-linked glycosylation in hypoxia-induced down-regulation of cell-surface MICA using osteosarcoma cells. MATERIALS AND METHODS:The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively. RESULTS:Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA. 2-Deoxy-D-glucose, which inhibits N-linked glycosylation, reduced the cell-surface expression of MICA under normoxia, while D-Mannose increased N-glycosylated MICA, increasing cell-surface MICA under hypoxia. Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation. CONCLUSION:The inhibition of Asn-N-linked glycosylation participates in hypoxia-induced down-regulation of cell-surface expression of MICA. |
巻・号 | 38(3) |
ページ | 1353-1359 |
公開日 | 2018-3-1 |
DOI | 10.21873/anticanres.12358 |
PII | 38/3/1353 |
PMID | 29491059 |
MeSH | Amino Acid Sequence Asparagine / genetics* Asparagine / metabolism Cell Hypoxia Cell Line, Tumor Cell Membrane / genetics Cell Membrane / metabolism Deoxyglucose / pharmacology Down-Regulation* Gene Expression Regulation, Neoplastic* Glycosylation / drug effects Histocompatibility Antigens Class I / genetics* Histocompatibility Antigens Class I / metabolism Humans Osteosarcoma / genetics Osteosarcoma / metabolism Osteosarcoma / pathology |
IF | 1.994 |
引用数 | 3 |
リソース情報 | |
ヒト・動物細胞 | HOS(RCB0992) |