| RRC ID |
52682
|
| 著者 |
Islam MS, Kaji N, Mikawa S, Yang Q, Kusabe M, Hori M, Ozaki H.
|
| タイトル |
Induction of myosin light chain kinase and CPI-17 by TGF-β accelerates contractile activity in intestinal epithelial cells.
|
| ジャーナル |
J Vet Med Sci
|
| Abstract |
Epithelial-mesenchymal transition (EMT) is an orchestral and functional change in epithelial cells. Many signaling pathways are involved in EMT, and transforming growth factor-beta (TGF-β) is considered to be one of the most important factors in induction of EMT. In this study, we treated the rat intestinal epithelial cell line (IEC-6) with TGF-β1 as a signaling stimulant. Gross analysis of IEC-6 cells showed typical characteristics of epithelial cells such as cuboidal morphology and cell-cell contact, whereas treatment with TGF-β1 (10 ng/ml-1) for 7 days produced robust, spindle-shaped morphology. Immunocytochemistry analysis showed distinct E-cadherin staining in IEC-6 cells, but weak and faint in EMT cells. EMT cells showed positive expression of α-SMA and tenascin-C but IEC-6 cells did not. Quantitative real-time PCR analysis showed that myosin light chain kinase and C-kinase potentiated protein phosphatase-1 inhibitor (CPI-17) mRNAs were significantly upregulated in EMT cells. Immunocytochemistry analysis also showed that EMT cells strongly expressed CPI-17 but IEC-6 cells did not. A collagen gel contraction assay revealed that EMT cells had greatly increased contraction compared with control cells. These results suggest that the increased contractile activity induced by TGF-β in EMT cells may be attributable to the upregulation of molecules responsible for myosin phosphorylation/de-phosphorylation.
|
| 巻・号 |
80(6)
|
| ページ |
977-984
|
| 公開日 |
2018-6-29
|
| DOI |
10.1292/jvms.17-0684
|
| PMID |
29695674
|
| PMC |
PMC6021892
|
| MeSH |
Animals
Cyclopropanes / metabolism*
Epithelial Cells
Epithelial-Mesenchymal Transition*
Indoles / metabolism*
Japan
Myosin-Light-Chain Kinase / metabolism*
Rats
Transforming Growth Factor beta1 / pharmacology*
|
| IF |
0.803
|
| 引用数 |
0
|
| リソース情報 |
| ヒト・動物細胞 |
IEC 6(RCB0993) |
