RRC ID |
52696
|
著者 |
Tsuchida A, Senda M, Ito A, Saito S, Kiso M, Ando T, Harduin-Lepers A, Matsuda A, Furukawa K, Furukawa K.
|
タイトル |
Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells.
|
ジャーナル |
Sci Rep
|
Abstract |
GalNAc-disialyl Lc4 (GalNAc-DSLc4) was reported as a novel antigen that associated with malignant features of renal cell cancers (RCCs). To clarify roles of GalNAc-DSLc4 in malignant properties of RCCs, we identified B4GalNAc-T2 as a responsible gene for the synthesis of GalNAc-DSLc4, and prepared stable transfectants of GalNAc-T2 cDNA using VMRC-RCW cells, resulting in the establishment of high expressants of GalNAc-DSLc4. They showed increased proliferation and invasion, and specific adhesion to laminin. In the transfectants, PI3K/Akt signals were highly activated by serum stimulation or adhesion to laminin. GalNAc-DSLc4 was co-localized in lipid rafts with integrin β1 and caveolin-1 in both immunoblotting of fractionated detergent extracts and immunocytostaining, particularly when stimulated with serum. Masking of GalNAc-DSLc4 with antibodies as well as PI3K inhibitor suppressed malignant properties of the transfectants. These results suggested that GalNAc-DSLc4 is involved in malignant properties of RCCs by forming a molecular complex with integrins in lipid rafts.
|
巻・号 |
8(1)
|
ページ |
7017
|
公開日 |
2018-5-4
|
DOI |
10.1038/s41598-018-25521-6
|
PII |
10.1038/s41598-018-25521-6
|
PMID |
29728594
|
PMC |
PMC5935701
|
MeSH |
Antigens, Neoplasm / immunology*
Carcinoma, Renal Cell / immunology*
Carcinoma, Renal Cell / metabolism
Cell Adhesion
Cell Line, Tumor
Cell Movement
Cell Proliferation
Glycolipids
Humans
Integrins / metabolism
Kidney Neoplasms / immunology*
Kidney Neoplasms / metabolism
Phosphorylation
Protein Transport
Proto-Oncogene Proteins c-akt / metabolism
Signal Transduction
|
IF |
3.998
|
引用数 |
1
|
リソース情報 |
ヒト・動物細胞 |
OS-RC-2(RCB0735)
RCC10RGB(RCB1151)
TUHR4TKB(RCB1198) |