論文 - 詳細
| RRC ID | 53102 |
|---|---|
| 著者 | Ranjan P, Kateriya S. |
| タイトル | Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae. |
| ジャーナル | BMC Res Notes |
| Abstract |
OBJECTIVE:The eukaryotic plasma membrane localized light-gated proton-pumping rhodopsins possesses great optogenetic applications for repolarization (silencing) of the neuronal activity simply by light illumination. Very few plasma membrane localized proton-pumping rhodopsins of a eukaryotic origin are known that have optogenetic potential. Our objective was to identify and characterize microbial rhodopsin of an eukaryotic origin that expresses on plasma membrane. The plasma membrane localized light-gated proton pump of an eukaryotic origin hold great promise to be used as an optogenetic tools for the neurobiology. RESULTS:Here, we had characterized the cellular expression and membrane localization of a new rhodopsin in Antarctican algae Coccomyxa subellipsoidea. It is the first algal ion pumping rhodopsin that localizes to the plasma membrane of the eukaryotic cells. Coccomyxa subellipsoidea rhodopsin exists in the monomeric and dimeric state both the in vivo and in vitro. The dimeric form of the Coccomyxa subellipsoidea rhodopsin is resistant to heat and detergent denaturants. |
| 巻・号 | 11(1) |
| ページ | 65 |
| 公開日 | 2018-1-24 |
| DOI | 10.1186/s13104-018-3181-4 |
| PII | 10.1186/s13104-018-3181-4 |
| PMID | 29361974 |
| PMC | PMC5781313 |
| MeSH | Algal Proteins / chemistry Algal Proteins / genetics Algal Proteins / metabolism* Amino Acid Sequence Antarctic Regions Cell Membrane / metabolism* Cell Membrane / radiation effects Chlorophyta / genetics Chlorophyta / metabolism* Immunoblotting Light Protein Multimerization Protein Stability Proton Pumps / genetics Proton Pumps / metabolism Rhodopsin / chemistry Rhodopsin / genetics Rhodopsin / metabolism* Sequence Homology, Amino Acid |
| リソース情報 | |
| 藻類 | NIES-2166 |