RRC ID |
539
|
著者 |
Ote M, Mita K, Kawasaki H, Kobayashi M, Shimada T.
|
タイトル |
Characteristics of two genes encoding proteins with an ADAM-type metalloprotease domain, which are induced during the molting periods in Bombyx mori.
|
ジャーナル |
Arch Insect Biochem Physiol
|
Abstract |
By microarray analyses, we identified two genes (BmADAMTS-1 and BmADAMTS-like) encoding a protein, which are induced during the pupal ecdysis in the wing discs of Bombyx mori; these genes are homologous to ADAMTS family members (a disintegrin and metalloproteinase domain, with thrombospondin type-1 repeats). A complete metal-binding motif of the ADAM-type metalloprotease domain (HEXXHXXGXXHD) was contained in both amino acid sequences. However, thrombospondin type 1 (TSP-1) repeats were observed only in BmADAMTS-1. The BmADAMTS-1 gene was expressed in the hemocyte and midgut of the larvae at day 2 of wandering stage (W2), and strongly induced during the pupal ecdysis in the hemolymph. The BmADAMTS-like gene was expressed in the epithelial tissues of the larvae at W2, and had expression peaks slightly later than the BmADAMTS-1 gene. Our results indicate that BmADAMTS-1 and BmADAMTS-like may cleave the extracellular matrix (ECM) in the degenerating and remodeling tissues during the molting periods.
|
巻・号 |
59(2)
|
ページ |
91-8
|
公開日 |
2005-6-1
|
DOI |
10.1002/arch.20059
|
PMID |
15898116
|
MeSH |
ADAM Proteins
ADAMTS1 Protein
Amino Acid Motifs / genetics
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Bombyx / genetics*
Bombyx / metabolism
DNA Primers
DNA, Complementary / genetics
Disintegrins / biosynthesis*
Disintegrins / genetics*
Disintegrins / metabolism
Enzyme Induction / genetics
Extracellular Matrix Proteins / genetics*
Extracellular Matrix Proteins / metabolism
Gene Components
Larva / metabolism
Metalloendopeptidases / biosynthesis*
Metalloendopeptidases / genetics*
Metalloendopeptidases / metabolism
Molecular Sequence Data
Molting / genetics
Molting / physiology*
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Thrombospondin 1 / genetics
|
IF |
1.536
|
引用数 |
5
|
WOS 分野
|
ENTOMOLOGY
PHYSIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
カイコ |
p50 |