| RRC ID |
54298
|
| 著者 |
Tanaka C, Itoh T, Iwasaki Y, Mizuno N, Nasuda S, Murai K.
|
| タイトル |
Direct interaction between VRN1 protein and the promoter region of the wheat FT gene.
|
| ジャーナル |
Genes Genet Syst
|
| Abstract |
The wheat florigen gene Wheat FLOWERING LOCUS T (WFT, which is identical to VRN3) is an integrator of the vernalization, photoperiod and autonomous pathways in wheat flowering. Many studies have indicated that VERNALIZATION 1 (VRN1) directly or indirectly up-regulates WFT expression in leaves. VRN1 encodes an APETALA1/FRUITFULL-like MADS box transcription factor that is up-regulated by vernalization and aging, leading to promotion of flowering. In this study, the VRN1 protein was expressed as a His-Tag fusion protein in Escherichia coli and used in an electrophoretic mobility shift assay (EMSA). The results from the EMSA indicated that the VRN1 protein directly binds to the CArG-box in the promoter region of WFT, suggesting the direct up-regulation of WFT by VRN1 in the leaves of wheat plants.
|
| 巻・号 |
93(1)
|
| ページ |
25-29
|
| 公開日 |
2018-7-13
|
| DOI |
10.1266/ggs.17-00041
|
| PMID |
29343669
|
| MeSH |
Cloning, Molecular
Gene Expression Regulation, Plant
MADS Domain Proteins / metabolism*
Plant Leaves / genetics
Plant Leaves / metabolism
Plant Proteins / genetics*
Plant Proteins / metabolism
Promoter Regions, Genetic
Protein Binding
Triticum / genetics
Triticum / metabolism*
Up-Regulation*
|
| IF |
0.917
|
| 引用数 |
5
|
| リソース情報 |
| コムギ |
|
