| RRC ID |
54731
|
| 著者 |
Calistri NL, Kimmerling RJ, Malinowski SW, Touat M, Stevens MM, Olcum S, Ligon KL, Manalis SR.
|
| タイトル |
Microfluidic active loading of single cells enables analysis of complex clinical specimens.
|
| ジャーナル |
Nat Commun
|
| Abstract |
A fundamental trade-off between flow rate and measurement precision limits performance of many single-cell detection strategies, especially for applications that require biophysical measurements from living cells within complex and low-input samples. To address this, we introduce 'active loading', an automated, optically-triggered fluidic system that improves measurement throughput and robustness by controlling entry of individual cells into a measurement channel. We apply active loading to samples over a range of concentrations (1-1000 particles μL-1), demonstrate that measurement time can be decreased by up to 20-fold, and show theoretically that performance of some types of existing single-cell microfluidic devices can be improved by implementing active loading. Finally, we demonstrate how active loading improves clinical feasibility for acute, single-cell drug sensitivity measurements by deploying it to a preclinical setting where we assess patient samples from normal brain, primary and metastatic brain cancers containing a complex, difficult-to-measure mixture of confounding biological debris.
|
| 巻・号 |
9(1)
|
| ページ |
4784
|
| 公開日 |
2018-11-14
|
| DOI |
10.1038/s41467-018-07283-x
|
| PII |
10.1038/s41467-018-07283-x
|
| PMID |
30429479
|
| PMC |
PMC6235965
|
| MeSH |
Animals
Cell Line
Cells, Cultured
Equipment Design
Humans
Mice
Microfluidic Analytical Techniques / instrumentation*
Microfluidic Analytical Techniques / methods*
Reproducibility of Results
Single-Cell Analysis / instrumentation*
Single-Cell Analysis / methods*
Tumor Cells, Cultured
|
| IF |
11.878
|
| 引用数 |
7
|
| リソース情報 |
| ヒト・動物細胞 |
Ba/F3(RCB0805) |
