Reference - Detail
| RRC ID | 55051 |
|---|---|
| Author | Fuwa K, Seki M, Hirata Y, Yanagihara I, Nakura Y, Takano C, Kuroda K, Hayakawa S. |
| Title | Rapid and simple detection of Ureaplasma species from vaginal swab samples using a loop-mediated isothermal amplification method. |
| Journal | Am J Reprod Immunol |
| Abstract |
PROBLEM:Ureaplasma species occasionally cause chorioamnionitis and premature labor. We developed a novel assay employing a loop-mediated isothermal amplification (LAMP) method to detect Ureaplasma parvum and Ureaplasma urealyticum. METHOD OF STUDY:Loop-mediated isothermal amplification primers were designed to amplify Ureaplasma-specific ureaseB genes. Four U. parvum strains, 5 U. urealyticum strains and 14 reference bacterial species were evaluated. Forty-six vaginal swab samples were analyzed by LAMP, culture, and PCR. RESULTS:Our LAMP primers were specific to each species and had no cross-reaction. Of 46 clinical specimens, the sensitivity, specificity, and positive and negative predictive values of the LAMP method were 100% (12/12), 100% (34/34), 100% (12/12), and 100% (34/34), respectively, whereas those of PCR were 66.7% (8/12), 100% (34/34), 100% (8/8), and 89.5% (34/38), respectively, compared to culture-based detection. CONCLUSION:The LAMP detection method outperformed the culture and PCR methods. Early detection enables appropriate antibiotic selection for improved prenatal outcomes. |
| Volume | 79(1) |
| Published | 2018-1-1 |
| DOI | 10.1111/aji.12771 |
| PMID | 29154392 |
| MeSH | Cell Culture Techniques Cells, Cultured Chorioamnionitis / diagnosis* Early Diagnosis Female Humans Nucleic Acid Amplification Techniques / methods* Polymerase Chain Reaction Predictive Value of Tests Pregnancy Sensitivity and Specificity Time Factors Ureaplasma / physiology* Ureaplasma Infections / diagnosis* Ureaplasma urealyticum / physiology* Vagina / physiology* |
| IF | 3.091 |
| Times Cited | 2 |
| Resource | |
| General Microbes | JCM 1232 JCM 11026 JCM 12093 JCM 1669 JCM 20624 |