| RRC ID |
55487
|
| 著者 |
Sun L, Lu Z, Li J, Sun F, Huang R.
|
| タイトル |
Comparative genomics and transcriptome analysis of Lactobacillus rhamnosus ATCC 11443 and the mutant strain SCT-10-10-60 with enhanced L-lactic acid production capacity.
|
| ジャーナル |
Mol Genet Genomics
|
| Abstract |
Mechanisms for high L-lactic acid production remain unclear in many bacteria. Lactobacillus rhamnosus SCT-10-10-60 was previously obtained from L. rhamnosus ATCC 11443 via mutagenesis and showed improved L-lactic acid production. In this study, the genomes of strains SCT-10-10-60 and ATCC 11443 were sequenced. Both genomes are a circular chromosome, 2.99 Mb in length with a GC content of approximately 46.8%. Eight split genes were identified in strain SCT-10-10-60, including two LytR family transcriptional regulators, two Rex redox-sensing transcriptional repressors, and four ABC transporters. In total, 60 significantly up-regulated genes (log2fold-change ≥ 2) and 39 significantly down-regulated genes (log2fold-change ≤ - 2) were identified by a transcriptome comparison between strains SCT-10-10-60 and ATCC 11443. KEGG pathway enrichment analysis revealed that "pyruvate metabolism" was significantly different (P < 0.05) between the two strains. The split genes and the differentially expressed genes involved in the "pyruvate metabolism" pathway are probably responsible for the increased L-lactic acid production by SCT-10-10-60. The genome and transcriptome sequencing information and comparison of SCT-10-10-60 with ATCC 11443 provide insights into the anabolism of L-lactic acid and a reference for improving L-lactic acid production using genetic engineering.
|
| 巻・号 |
293(1)
|
| ページ |
265-276
|
| 公開日 |
2018-2-1
|
| DOI |
10.1007/s00438-017-1379-0
|
| PII |
10.1007/s00438-017-1379-0
|
| PMID |
29159508
|
| MeSH |
Gene Expression Regulation, Bacterial
Genome, Bacterial
Genomics*
Lactic Acid / biosynthesis*
Lacticaseibacillus rhamnosus / genetics*
Lacticaseibacillus rhamnosus / metabolism
Metabolic Engineering
Mutation
Pyruvic Acid / metabolism
Transcriptome / genetics*
|
| IF |
2.879
|
| 引用数 |
2
|
| リソース情報 |
| 一般微生物 |
JCM 1553 |
