RRC ID |
56027
|
著者 |
Schoonenberg VAC, Cole MA, Yao Q, Macias-Treviño C, Sher F, Schupp PG, Canver MC, Maeda T, Pinello L, Bauer DE.
|
タイトル |
CRISPRO: identification of functional protein coding sequences based on genome editing dense mutagenesis.
|
ジャーナル |
Genome Biol
|
Abstract |
CRISPR/Cas9 pooled screening permits parallel evaluation of comprehensive guide RNA libraries to systematically perturb protein coding sequences in situ and correlate with functional readouts. For the analysis and visualization of the resulting datasets, we develop CRISPRO, a computational pipeline that maps functional scores associated with guide RNAs to genomes, transcripts, and protein coordinates and structures. No currently available tool has similar functionality. The ensuing genotype-phenotype linear and three-dimensional maps raise hypotheses about structure-function relationships at discrete protein regions. Machine learning based on CRISPRO features improves prediction of guide RNA efficacy. The CRISPRO tool is freely available at gitlab.com/bauerlab/crispro .
|
巻・号 |
19(1)
|
ページ |
169
|
公開日 |
2018-10-19
|
DOI |
10.1186/s13059-018-1563-5
|
PII |
10.1186/s13059-018-1563-5
|
PMID |
30340514
|
PMC |
PMC6195731
|
MeSH |
CRISPR-Cas Systems / genetics*
Cell Line
Gene Editing*
Genome*
Humans
Molecular Sequence Annotation
Mutagenesis / genetics*
Open Reading Frames / genetics*
Protein Structure, Secondary
RNA, Guide, Kinetoplastida / genetics
RNA, Messenger / genetics
RNA, Messenger / metabolism
|
IF |
14.028
|
引用数 |
7
|
リソース情報 |
ヒト・動物細胞 |
HUDEP-2(RCB4557) |