論文 - 詳細
| RRC ID | 5698 |
|---|---|
| 著者 | Niwa R, Nagata-Ohashi K, Takeichi M, Mizuno K, Uemura T. |
| タイトル | Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin. |
| ジャーナル | Cell |
| Abstract |
The ADF (actin-depolymerizing factor)/cofilin family is a stimulus-responsive mediator of actin dynamics. In contrast to the mechanisms of inactivation of ADF/cofilin by kinases such as LIM-kinase 1 (LIMK1), much less is known about its reactivation through dephosphorylation. Here we report Slingshot (SSH), a family of phosphatases that have the property of F actin binding. In Drosophila, loss of ssh function dramatically increased levels of both F actin and phospho-cofilin (P cofilin) and disorganized epidermal cell morphogenesis. In mammalian cells, human SSH homologs (hSSHs) suppressed LIMK1-induced actin reorganization. Furthermore, SSH and the hSSHs dephosphorylated P cofilin in cultured cells and in cell-free assays. Our results strongly suggest that the SSH family plays a pivotal role in actin dynamics by reactivating ADF/cofilin in vivo. |
| 巻・号 | 108(2) |
| ページ | 233-46 |
| 公開日 | 2002-1-25 |
| DOI | 10.1016/s0092-8674(01)00638-9 |
| PII | S0092867401006389 |
| PMID | 11832213 |
| MeSH | Actin Depolymerizing Factors Actins / metabolism* Amino Acid Sequence Animals Cell Line Cell-Free System Destrin Drosophila / anatomy & histology Drosophila / genetics Drosophila / metabolism* Drosophila Proteins* Embryonic Structures / anatomy & histology Embryonic Structures / metabolism Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Humans Immunohistochemistry LIM-Homeodomain Proteins Microfilament Proteins / metabolism* Molecular Sequence Data Phosphoprotein Phosphatases Phosphoric Monoester Hydrolases / genetics Phosphoric Monoester Hydrolases / metabolism* Phosphorylation Protein Binding Protein Serine-Threonine Kinases / metabolism Sequence Alignment Transcription Factors Zyxin |
| IF | 38.637 |
| 引用数 | 475 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | |