| RRC ID |
57156
|
| 著者 |
Peiris H, Park S, Louis S, Gu X, Lam JY, Asplund O, Ippolito GC, Bottino R, Groop L, Tucker H, Kim SK.
|
| タイトル |
Discovering human diabetes-risk gene function with genetics and physiological assays.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Developing systems to identify the cell type-specific functions regulated by genes linked to type 2 diabetes (T2D) risk could transform our understanding of the genetic basis of this disease. However, in vivo systems for efficiently discovering T2D risk gene functions relevant to human cells are currently lacking. Here we describe powerful interdisciplinary approaches combining Drosophila genetics and physiology with human islet biology to address this fundamental gap in diabetes research. We identify Drosophila orthologs of T2D-risk genes that regulate insulin output. With human islets, we perform genetic studies and identify cognate human T2D-risk genes that regulate human beta cell function. Loss of BCL11A, a transcriptional regulator, in primary human islet cells leads to enhanced insulin secretion. Gene expression profiling reveals BCL11A-dependent regulation of multiple genes involved in insulin exocytosis. Thus, genetic and physiological systems described here advance the capacity to identify cell-specific T2D risk gene functions.
|
| 巻・号 |
9(1)
|
| ページ |
3855
|
| 公開日 |
2018-9-21
|
| DOI |
10.1038/s41467-018-06249-3
|
| PII |
10.1038/s41467-018-06249-3
|
| PMID |
30242153
|
| PMC |
PMC6155000
|
| MeSH |
Animals
Carrier Proteins / metabolism*
Diabetes Mellitus, Type 2 / genetics*
Drosophila
Exocytosis
Gene Expression Profiling
Genes, Insect*
Genetic Predisposition to Disease
Humans
Insulin Secretion / genetics
Islets of Langerhans / metabolism*
Nuclear Proteins / metabolism*
Repressor Proteins
Risk Assessment
|
| IF |
11.878
|
| 引用数 |
9
|
| リソース情報 |
| ショウジョウバエ |
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