| RRC ID |
57311
|
| 著者 |
Yamamura ET.
|
| タイトル |
Construction of Rhodococcus expression vectors and expression of the aminoalcohol dehydrogenase gene in Rhodococcus erythropolis.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
NADP+-dependent aminoalcohol dehydrogenase (AADH) of Rhodococcus erythropolis MAK154 produces double chiral aminoalcohols, which are used as pharmaceuticals. However, the genetic manipulation of Rhodococcus strains to increase their production of such industrially important enzymes is not well studied. Therefore, I aimed to construct Rhodococcus expression vectors, derived from the Rhodococcus-Escherichia coli shuttle vector pRET1102, to express aadh. The plasmid pRET1102 could be transformed into many actinomycete strains, including R. erythropolis. The transformation efficiency for a species closely related to R. erythropolis was higher than that for other actinomycete strains. Promoters of various strengths, hsp, 1200rep, and TRR, were obtained from Gram-positive bacteria. The activity of TRR was stronger than that of hsp and 1200rep. The aadh-expressing plasmid pRET1172 with TRR could be transformed into many actinomycete strains to increase their AADH production. The Rhodococcus expression vector, pRET11100, constructed by removing aadh from the pRET1172 plasmid may be useful for bioconversion.
|
| 巻・号 |
82(8)
|
| ページ |
1396-1403
|
| 公開日 |
2018-8-1
|
| DOI |
10.1080/09168451.2018.1463154
|
| PMID |
29673281
|
| MeSH |
Amino Alcohols / metabolism*
Biotransformation
Escherichia coli / genetics
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Genes, Bacterial
Genetic Vectors*
Oxidoreductases / genetics*
Phylogeny
Plasmids
Promoter Regions, Genetic
Rhodococcus / classification
Rhodococcus / genetics*
|
| IF |
1.297
|
| 引用数 |
3
|
| リソース情報 |
| 一般微生物 |
JCM 9703, JCM 3203, JCM 9919, JCM 13270, JCM 10743, JCM 10087, JCM 11308, JCM 11374, JCM 13366, JCM 3376, JCM 13011, JCM 13396, JCM 10940, JCM 3231, JCM 3199, JCM 3205, JCM 11615 |
