| RRC ID |
57517
|
| 著者 |
Komine-Abe A, Nagano-Shoji M, Kubo S, Kawasaki H, Yoshida M, Nishiyama M, Kosono S.
|
| タイトル |
Effect of lysine succinylation on the regulation of 2-oxoglutarate dehydrogenase inhibitor, OdhI, involved in glutamate production in Corynebacterium glutamicum.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
In Corynebacterium glutamicum, the activity of the 2-oxoglutarate dehydrogenase (ODH) complex is negatively regulated by the unphosphorylated form of OdhI protein, which is critical for L-glutamate overproduction. We examined the potential impact of protein acylation at lysine (K)-132 of OdhI in C. glutamicum ATCC13032. The K132E succinylation-mimic mutation reduced the ability of OdhI to bind OdhA, the catalytic subunit of the ODH complex, which reduced the inhibition of ODH activity. In vitro succinylation of OdhI protein also reduced the ability to inhibit ODH, and the K132R mutation blocked the effect. These results suggest that succinylation at K132 may attenuate the OdhI function. Consistent with these results, the C. glutamicum mutant strain with OdhI-K132E showed decreased L-glutamate production. Our results indicated that not only phosphorylation but also succinylation of OdhI protein may regulate L-glutamate production in C. glutamicum.
|
| 巻・号 |
81(11)
|
| ページ |
2130-2138
|
| 公開日 |
2017-11-1
|
| DOI |
10.1080/09168451.2017.1372182
|
| PMID |
28899215
|
| MeSH |
Amino Acid Sequence
Corynebacterium glutamicum / enzymology
Corynebacterium glutamicum / metabolism*
Enzyme Inhibitors / pharmacology*
Glutamic Acid / biosynthesis*
Ketoglutarate Dehydrogenase Complex / antagonists & inhibitors*
Ketoglutarate Dehydrogenase Complex / chemistry
Ketoglutarate Dehydrogenase Complex / genetics
Ketoglutarate Dehydrogenase Complex / metabolism*
Lysine / metabolism*
Models, Molecular
Mutation
Phosphorylation
Protein Domains
Protein Processing, Post-Translational*
Succinic Acid / metabolism
|
| IF |
1.297
|
| 引用数 |
3
|
| リソース情報 |
| 一般微生物 |
JCM 1318 |
