論文 - 詳細
| RRC ID | 57764 |
|---|---|
| 著者 | Meng D, Li J, Li H, Wang K. |
| タイトル | Salvianolic acid B remits LPS-induced injury by up-regulating miR-142-3p in MH7A cells. |
| ジャーナル | Biomed Pharmacother |
| Abstract |
BACKGROUND:Rheumatoid arthritis (RA) is a common inflammatory disease, which significantly reduces the quality of life and increases the risk of cardiovascular and cerebrovascular diseases. The present work studied the therapeutic potency of Salvianolic acid B (Sal-B) for RA and revealed one of the possible underlying mechanisms. METHODS:Human rheumatoid fibroblast-like synoviocytes (MH7 A) were treated with Sal-B before, during or after lipopolysaccharide (LPS) stimulation. CCK-8 assay, Annexin V-FITC/PI double-staining, RT-qPCR, Western blotting and ELISA were carried out to measure the changes of cell viability, apoptosis, and the release of pro-inflammatory cytokines. Next, the involvement of miR-142-3p and related signaling pathways in Sal-B-mediated protection was studied. RESULTS:Sal-B (10 μM) treatment significantly ameliorated LPS injury to MH7 A cells, as cell viability was increased, expression of p53 and p21 was repressed, apoptosis was inhibited, and the release of MCP-1, IL-6 and TNF-α was reduced. However, Sal-B (10 μM) treated alone has no impacts on MH7 A cells in the abovementioned aspects. miR-142-3p was down-regulated by LPS stimulation, while was up-regulated by treatment of Sal-B. Rescue assay results showed that Sal-B did not remit LPS injury when miR-142-3p was silenced. And also, the inhibitory effects of Sal-B on NF-κB and JNK pathways were abolished by miR-142-3p silence. CONCLUSION:Sal-B could protect against and reverse LPS-induced injury in MH7 A cells, showing anti-apoptotic and anti-inflammatory capacities. The anti-RA potential of Sal-B might be via up-regulating miR-142-3p, and subsequently modulating NF-κB and JNK pathways. |
| 巻・号 | 115 |
| ページ | 108876 |
| 公開日 | 2019-7-1 |
| DOI | 10.1016/j.biopha.2019.108876 |
| PII | S0753-3322(19)30458-5 |
| PMID | 31022596 |
| MeSH | Benzofurans / chemistry Benzofurans / pharmacology* Caspase 3 / genetics Caspase 3 / metabolism Caspase 9 / genetics Caspase 9 / metabolism Cell Line Cell Survival / drug effects Cyclin-Dependent Kinase Inhibitor p21 / genetics Cyclin-Dependent Kinase Inhibitor p21 / metabolism Cytokines / genetics Cytokines / metabolism Cytoprotection / drug effects Gene Expression Regulation / drug effects* Humans Lipopolysaccharides / toxicity* MicroRNAs / genetics MicroRNAs / metabolism* Molecular Structure RNA, Messenger / genetics RNA, Messenger / metabolism Synoviocytes / drug effects* Synoviocytes / metabolism Tumor Suppressor Protein p53 / genetics Tumor Suppressor Protein p53 / metabolism Up-Regulation |
| IF | 4.545 |
| 引用数 | 2 |
| リソース情報 | |
| ヒト・動物細胞 | MH7A(RCB1512) |