論文 - 詳細
| RRC ID | 58818 |
|---|---|
| 著者 | Yashiro M, Ohya M, Mima T, Nakashima Y, Kawakami K, Sonou T, Tatsuta K, Yamano Y, Negi S, Shigematsu T. |
| タイトル | Excessive ADAM17 activation occurs in uremic patients and may contribute to their immunocompromised status. |
| ジャーナル | Immun Inflamm Dis |
| Abstract |
INTRODUCTION:We previously reported that fibroblast growth factor 23 (FGF23)-klotho signaling plays a role in B cell immunity. Despite high serum levels of FGF23, a decline in immunity is frequently observed in patients on hemodialysis (HD); thus, abnormalities in the FGF23-klotho signaling pathway in immune cells may occur in these patients. METHODS:We analyzed the number of klotho-positive cells in peripheral blood mononuclear cells from 10 male and 6 female patients on HD and 5 healthy male subjects using flow cytometry. We analyzed the abundance of cleaved klotho protein in the murine B cell line, A20, and in the serum of HD patients and healthy subjects (HS) using flow cytometry and Western blotting. The serum level of A disintegrin and metalloprotease 17 (ADAM17) was measured in HD patients and HS using enzyme-linked immunosorbent assay. RESULTS:The number of klotho-positive B cells was reduced in HD patients. Serum ADAM17 was responsible for the reduction in klotho, as a specific ADAM17 inhibitor reversed this change. The total serum levels of ADAM17 were similar in HD patients and HS; however, activated ADAM17 was increased in the serum of HD patients. CONCLUSIONS:We concluded that abnormal ADAM17 activation could contribute to the immunocompromised status in patients on HD, in line with the reported role of ADAM17 as an anti-inflammatory and immunosuppressive factor. |
| 巻・号 | 8(2) |
| ページ | 228-235 |
| 公開日 | 2020-6-1 |
| DOI | 10.1002/iid3.298 |
| PMID | 32180373 |
| PMC | PMC7212198 |
| MeSH | ADAM17 Protein / blood* ADAM17 Protein / genetics Aged Aged, 80 and over Animals Case-Control Studies Cell Line Female Fibroblast Growth Factor-23 Fibroblast Growth Factors / blood* Fibroblast Growth Factors / genetics Glucuronidase / blood* Glucuronidase / genetics Humans Immunocompromised Host Klotho Proteins Leukocytes, Mononuclear / metabolism* Male Mice Middle Aged Renal Dialysis Renal Insufficiency, Chronic / blood Renal Insufficiency, Chronic / genetics* Renal Insufficiency, Chronic / therapy Uremia / blood Uremia / genetics |
| IF | 2.5 |
| 引用数 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | A20(RCB2745) |