| RRC ID |
58821
|
| 著者 |
Kamiya Y, Kikuchi T, Goto H, Okabe I, Takayanagi Y, Suzuki Y, Sawada N, Okabe T, Suzuki Y, Kondo S, Hayashi JI, Mitani A.
|
| タイトル |
IL-35 and RANKL Synergistically Induce Osteoclastogenesis in RAW264 Mouse Monocytic Cells.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Interleukin (IL)-35 is an immunosuppressive cytokine mainly produced by regulatory T cells. IL-35 mediates immunological functions by suppressing the inflammatory immune response. However, the role of IL-35 in bone-destructive diseases remains unclear, especially in terms of osteoclastogenesis. Therefore, the current study investigated the synergistic effect of IL-35 on osteoclastogenesis that is involved the pathogeneses of periodontitis and rheumatoid arthritis. Osteoclastic differentiation and osteoclastogenesis of RAW264 (RAW) cells induced by receptor activator of nuclear factor (NF)-κB ligand (RANKL) and IL-35 were evaluated by tartrate-resistant acid phosphate staining, hydroxyapatite resorption assays, and quantitative polymerase chain reaction. The effect of IL-35 on RANKL-stimulated signaling pathways was assessed by Western blot analysis. Costimulation of RAW cells by RANKL and IL-35 induced osteoclastogenesis significantly compared with stimulation by RANKL alone. Phosphorylations of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase tended to be increased by RANKL and IL-35 compared with RANKL or IL-35 alone. Additionally, the osteoclastogenesis induced by RANKL and IL-35 was suppressed by inhibition of ERK. In this study, IL-35 and RANKL induced osteoclastogenesis synergistically. Previous reports have shown that IL-35 suppresses the differentiation of osteoclasts. Therefore, IL-35 might play dual roles of destruction and protection in osteoclastogenesis.
|
| 巻・号 |
21(6)
|
| 公開日 |
2020-3-18
|
| DOI |
10.3390/ijms21062069
|
| PII |
ijms21062069
|
| PMID |
32197293
|
| PMC |
PMC7139320
|
| MeSH |
Animals
Cell Differentiation / drug effects*
Interleukins / agonists
Interleukins / pharmacology*
MAP Kinase Signaling System / drug effects*
Mice
Monocytes / cytology
Monocytes / metabolism*
Osteoclasts / cytology
Osteoclasts / metabolism*
RANK Ligand / agonists
RANK Ligand / pharmacology*
RAW 264.7 Cells
|
| IF |
4.556
|
| 引用数 |
0
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
